【摘要】 目的:通过检测大鼠全脑缺血后再灌注纹状体内皮素(ET)、降钙素基因相关肽(CGRP)的表达和丙二醛(MDA)的含量变化,探讨ET和CGRP与脑缺血再灌注后神经元损伤的关系及褪黑激素(MT)对缺血再灌注纹状体ET、CGRP表达的影响。方法:于大鼠“四动脉结扎法”全脑缺血(30 m in)再灌注后第0、1、2、6 h分别腹腔注射褪黑激素2.5 mg/kg和10 mg/kg两个剂量,各组大鼠再灌注24 h后测定MDA含量,并用放射免疫法检测纹状体ET和CGRP含量变化。结果:MT 2.5 mg/kg和10 mg/kg两个剂量于大鼠全脑缺血(30 m in)再灌注后第0、1、2、6 h分别腹腔注射可降低再灌注24 h大鼠纹状体ET的表达,加强CGRP的表达,并降低了MDA的含量。结论:MT可能通过影响再灌注过程中ET和CGRP的表达,降低缺血神经元脂质过氧化反应,从而改善缺血再灌注神经元的延迟性损伤。更多还原

【Abstract】 Objective: By detecting the change of ET-1,CGRP and MDA concentrations in the striatum of rat after the global ischemia and reperfusion,to study mechanism and the protective role of MT for global ischemia and reperfusion injury.Methods: MT was injected intraperitoneally at 0 h,1 h,2 h,6 h after ischemia(30 min) induced by the "occlusion of four arteries",2.5 mg/kg or 10 mg/kg each time,respectively.Researching the changes of ET and CGRP with radioimmunoassey;the changes of MDA concentration 24 hour after reperfusion. Results: At these does,MT could decrease ET expression but increase CGRP expression,decrease the content of MDA at 24 h after global ischemia(30 min)/reperfusion. Conclusion: MT could improve delayed injury of neuron after reperfusion with influence expression of ET and CGRP,decrease the degree of lipids-peroxid,as well as.更多还原