【摘要】 目的:研究心力衰竭(CHF)大鼠吸入磷酸二酯酶Ⅲ抑制剂———米力农后血流动力学指标变化。方法:设充血性心力衰竭(CHF)组:雄性SD大鼠24只(100±8)g,在第二、三肋间开胸,用1 mm肽夹钳夹主动脉,造成主动脉缩窄,8~10周后,造成CHF,大鼠至(350±10)g,随机分为①吸入NaC l 3 m in,②吸入米力农(1 mg/m l)3 m in后予第二次开胸。正常对照组:雄性SD大鼠24只(350±10)g,,随机分为①吸入NaC l 3 m in,②吸入米力农(1 mg/m l)3m in。两组大鼠均予颈动、静脉置管,开胸后分别给予肺动脉和左房置管,分别测量平均动脉压(MAP)、肺动脉压(PAP)、左房压(LAP)、中心静脉压(CVP)和心排出量(CO),并计算肺血管阻力(PVR)、外周血管阻力(SVR)、总血管阻力(TVR)、PVR/SVR,测量血气和称量肺干湿重比(W/D)和心脏重量和体重比(HW/BW)。结果:①CHF大鼠吸NaC l前后PVR/SVR无明显改变;②CHF大鼠吸米力农前后PVR/SVR明显下降,P<0.05;③对照组吸米力农前后PVR/SVR无明显改变;④CHF大鼠HW/BW明显高于正常对照组大鼠,P<0.05;CHF大鼠肺W/D明显高于正常对照组大鼠,P<0.05。结论:米力农吸入治疗明显降低CHF大鼠PVR/SVR,吸入治疗不改变体循环血压而降低肺动脉压,这种方法可作为新的治疗CHF的方法用于临床。更多还原

【Abstract】 Objective: To study changes in hemodynamics of milrinon inhalation treatment congestive heart failure(CHF) rats.Methods: CHF rats group: male 24 SD rats(100±8)g,bw,in the first open chest operation first open ehest operation supracoronary aortic banding with titanium clip was giving,progressive development of CHF over 8~10 weeks,the rats weight arrived(350±10)g;In the second operation,put the catheter in pulmonary artery and left atrium,randomly divided two groups with each group 12 rats: group 1 inhalation NaCl,3 min,group 2 inhalation milrinone(1 mg/ml),3 min.Control group:normal male 24 SD rats(350±10)g,bw,randomly divided two groups, group 1 inhalation NaCl,3 min,group 2.inhalation milrinone(1 mg/ml),3 min,and were put the catheter into pulmonary artery and left atrium too.Both groups we measured MAP、 PAP、 LAP、 CVP CO and calculated PVR、 SVR、 TVR、 PVR/SVR.We measured blood gas and calculated the ratio of wet/dry lung weight(W/D),the ratio of heart/body weight(HW/BW).Results: ① In CHF rats before and after inhalation NaCl the ratio PVR/SVR had no significant change;② In CHF rats before and after inhalation mirinone the ratio PVR/SVR had significant decrease,P<0.05;③ In control group rats before and after inhalation mirinone the ratio PVR/SVR had no significant change;④ In CHF rats the ratio of HW/BW was significantly increased P<0.05,and the ratio of lung W/D was significantly increased too,P<0.05.Conclusion: Milrinon inhalation treatment decreased the ratio PVR/SVR in CHF.this treatment was a new trials to treating pulmonary venous hypertension.更多还原