【摘要】 用与牛血清蛋白(BSA)交联的毒死蜱人工抗原(CHBu-BSA)免疫的BALB/C鼠脾细胞与SP2/0鼠骨髓瘤细胞融合,经筛选和克隆,得到了1株能稳定分泌毒死蜱抗体的单克隆细胞株(1G10),1G10的抗体类型及亚类均为IgG1,其轻链为κ链.制备单克隆抗体腹水,腹水的间接ELISA效价在1×10-6以上.该单克隆抗体与毒死蜱特异性结合反应的50%抑制质量浓度为80.8μg?L-1,与其他毒死蜱结构类似物无交叉反应.更多还原

【Abstract】 One hybridoma cell line（1G10） secreting monoclonal antibody（McAb） against chlorpyrifos was produced by fusing mouse myeloma cells（SP2/0） with spleen cells from BALB/C which were immunized by the artificial antigen conjugated with bovine serum albumin（BSA）.Isotype and subclass of the monoclonal cell（1G10） was classified as IgG1.The light chain of the McAb was indentified to be κ.Its titres of ascitic fluids were up to 1×10^-6 by indirect ELISA.The inhibition rate of 50% of the McAb with the insecticide chlorpyrifos detected by ELISA was 80.8 μg·L^-1,the McAb could specifically react with chlorpyrifos but no cross-reactivity to chlorpyrigos analogues.更多还原