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利用ATP生物发光法测定西湖水体微生物量

Determination of micro-biomass in West Lake waters by ATP bioluminescence method

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【作者】 叶树明; 楼凯凯; 杨俊毅; 张东声; 陈杭;

【Author】 YE Shu-ming~(1,2),LOU Kai-kai~2,YANG Jun-yi~3,ZHANG Dong-sheng~3,CHEN Hang~1(1.College of Biomedical Engineering and Instrument Science,Zhejiang University,Hangzhou 310027,China;(2.College) of Life Sciences,Zhejiang University,Hangzhou 310027,China;3.Lab of Regional Oceanography,Second Institute of Oceanography of SOA,Hangzhou 310012,China)

【机构】 浙江大学生物医学工程与仪器科学学院; 浙江大学生命科学学院; 国家海洋局第二海洋研究所区域海洋学开放实验室; 浙江大学生物医学工程与仪器科学学院 浙江杭州310027; 浙江杭州310027; 浙江杭州310012;

【摘要】 介绍了ATP生物发光法的基本原理和测量方法,将该方法应用于西湖水体微生物量的实际测量,得到西湖水体微生物浓度为106~107个?mL-1,总体分布为近岸高于湖心,夏季高于春季,旅游、垂钓等人为活动对西湖水体微生物的分布有较大影响,另外,饭店养鱼池附近的微生物浓度较高,但对西湖主体并没有明显的影响,引水工程对西湖水体有一定的稀释作用,有利于净化水质.

【Abstract】 The principle of ATP bioluminescence assay and the measurement method were introduced.The method was applied to the measurement of micro-biomass in West Lake.The concentration of microorganisms in West Lake was 106-107cells·mL-1.In general,the concentration near bank was higher than that in the center of lake,and the concentration in summer was higher than that in spring.Human activities,such as tourism and angling,great influenced the microbial distribution in the lake.Moreover,the concentration of microorganisms in the vicinity of fishpond of a restaurant was relatively high,however,it had no obvious influence on the main body of lake.The import of pure water source was beneficial to the purity of water.

【关键词】 ATP; 生物发光法; 西湖; 微生物量;
【Key words】 ATP; bioluminescence; West Lake; micro-biomass;
【基金】 国家自然科学基金重点资助项目(40327001);浙江省科技计划资助项目(20040809135741)
  • 【文献出处】 浙江大学学报(农业与生命科学版) ,Journal of Zhejiang University(Agriculture and Life Sciences) , 编辑部邮箱 ,2006年05期
  • 【分类号】Q93-33
  • 【被引频次】20
  • 【下载频次】467
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