【摘要】 比较研究了6株球孢白僵菌Beuaveria bassiana(Bb)、5株玫烟色拟青霉Paecilomycesfumosoroseus(Pf)和3株金龟子绿僵菌Metarhizium anisopliae(Ma)孢子类疏水蛋白(HLP)的含量和组分.结果表明HLP含量因菌种和菌株不同而呈现显著差异,其中Bb2861孢子HLP含量最高达23.4μg?mg-1,而Ma297的最低仅5.0μg?mg-1.HLP组分在三种生防真菌间差异明显,球孢白僵菌(12.0、15.0和17.5 kDa)和玫烟色拟青霉(15.0和17.5 kDa)的种内差异不明显.但是,金龟子绿僵菌的HLP组分种内差异较大,Ma2125主要包括17.0和14.5 kDa条带,Ma0201主要包括19.0和13.5 kDa,而Ma297主要条带仅为13.5 kDa.在对Bb2860不同菌体结构的比较研究中,发现其气生分生孢子、气生菌丝和液生菌丝的HLP含量分别为26.3、18.2和16.5μg?mg-1,相互间差异显著,且均含有15.0和17.5 kDa的主要条带.害虫生防真菌孢子HLP含量及组分的初步探明为研究其生物学功能与潜在应用奠定了基础.更多还原

【Abstract】 Contents of hydrophobin-like proteins（HLP） extracted from aerial conidia of 14 isolates of Beauveria bassiana,Paecilomyces fumosoroseus and Metarhizium anisopliae varied significantly among the three fungal species and their isolates.Of those,the maximal HLP content in B.bassiana 2861 conidia was up to 23.4 μg·mg^-1,3.7 times greater than the minimal level of M.anisopliae 297(5.0 （μg·）mg^-1).Based on SDS-PAGE analysis,the HLP components differed among the three fungal species with the major molecules of 12.0,15.0 and 17.5 kDa in 6 B.bassiana isolates and of 15.0 and 17.5 kDa in 5 P.fumosoroseus isolates.However,the HLP components were more variable within M. anisopliae with the molecules of 17.0 and 14.5 kDa in the isolate Ma2125,19.0 and 13.5 kDa in Ma0201,and only 13.5 kDa in Ma297.Moreover,the aerial conidia,aerial mycelia and submerged mycelia of B.bassiana 2860 isolate grown on Sabouraud dextrose medium with or without agar differed significantly in their HLP contents(26.3,18.2 and 16.5 μg·mg^-1 respectively),which consistently consisted of the molecules of 15.0 and 17.5 kDa.The inter-and intraspecific variations in HLP contents and components are discussed in association with the understanding of their possible role in ecological adaptation and formulation enhancement.更多还原