【摘要】 构建亚非马蜂和额斑黄胡蜂前溶血肽原基因的重组供体质粒pBacHT-PhPPM和pBacHT-VmPPM,转化到穿梭载体Bacmid的受体菌DH10Bac中,得重组穿梭载体Bacmid-PhPPM和Bacmid-VmPPM,再用Lipofectin介导其DNA转染粉纹夜蛾细胞系Tn-5B1-4,最后观察细胞表达时相动态.经ELISA法证实,胞内表达产物具有良好的抗原性,证明二种重组病毒Bacmid-PhPPM和Bacmid-VmPPM已在昆虫细胞中得到了表达.更多还原

【Abstract】 Two cDNA fragments encoding prepromelittin of two vespoidea species,female Polistes hebraeus and Vespula maculifrons were obtained by restriction enzyme digestion from the recombinant plasmids and inserting into the multiple cloning sites of the pBacHTb to construct recombinant donor plasmids,which were used to transpose to the target Bacmids in E.coli(DH10) by Tn7 transposition function.Then these two recombinant Bacmids,Bacmid-PhPPM and Bacmid-VmPPM,were transfected into Tn-5B1-4 cells of the cabbage looper,Trichoplusia ni,mediated by Lipofectin.The expressed proteins detected by ELISA indicate that the recombinant Bacmids have been expressed in Tn-5B1-4 cells.更多还原