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转BAR基因水稻的抗性遗传、生理特性和农艺性状研究

Resistant inheritance,physiological and agricultural characters of BAR-transgenic rice.

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【作者】 段发平; 郑枫; 段俊; 黎垣庆; 梁承邺;

【Author】 DUAN Fa-ping,ZHENG Feng,DUAN Jun,LI Yuan-qing,LIANG Cheng-ye(South China Botanic Garden,Chinese Academy of Sciences,Guangzhou 510650,China)

【机构】 中国科学院华南植物园; 中国科学院华南植物园 广州510650; 广州510650;

【摘要】 为了明确BAR基因对水稻的影响,对转BAR基因的Bengal HU-10、Bengal HC-11、CypressPB-6、Cypress PB-13的抗性遗传、生理特性和农艺性状进行了研究,结果表明,它们对除草剂Liberty的抗性均受一对显性核基因控制,在这4个实验材料中,BAR基因在染色体上的整合位置互不等位,株高、生育期和每穗粒数等农艺性状也不相同.转BAR基因水稻与原亲本比较,叶片中叶绿素含量、光合速率和叶绿素荧光参数、GS酶活性等均未有明显差异,但用Liberty处理2 d后,上述生理性状变化明显,且在这4个转BAR基因材料之间也有显著差异,说明与BAR基因的插入位置有关.

【Abstract】 In order to investigate the effect of BAR gene on rice,the characteristics of inheritance,physiology and agriculture of 4 BAR-transgenic rice(Bengal HU-10,Bengal HC-11,Cypress PB-6,Cypress PB-13) were studied.The results indicated that the resistance of this 4 BAR-transgenic rice to herbicide(Liberty) was controlled by a single dominant nuclear gene.The inserted locus of BAR gene and agricultural properties including plant height,growth period and grain number per panicle were different among the 4 BAR-transgenic rice.There were no evident differences in chlorophyll content,photosynthetic rate,parameters of chlorophyll fluorescence and glutamine synthetase activity etc in leaves between BAR-transgenic rice and the original parents.However,the above physiological characters changed significantly and were significantly different between each BAR-transgenic rice in leaves 2 days later after spraying Liberty.The above results showed that the change of physiological characters was related to the inserted locus of BAR gene.

【基金】 广州市科技基金资助项目(2002J1-C0231)
  • 【文献出处】 浙江大学学报(农业与生命科学版) ,Journal of Zhejiang University(Agriculture and Life Sciences) , 编辑部邮箱 ,2006年04期
  • 【分类号】S511
  • 【被引频次】16
  • 【下载频次】254
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