【摘要】 一株4-氯硝基苯(4-CNB)的完全降解菌Pseudomonas putidaZWL73能利用3-羟基苯甲酸(3-HBA)为惟一碳源和能源生长,而且ZWL73在对3-HBA降解的过程中,有中间产物龙胆酸生成;酶学分析的分光光度法证实,ZWL73通过GSH依赖型的龙胆酸途径代谢3-HBA,并且该途径需经3-HBA或龙胆酸诱导.更多还原

【Abstract】 Pseudomonas putida ZWL73,which completely degrades 4-chloronitrobenzene(4-CNB),can grow on the media with 3-hydroxybenzoate(3-HBA) as a sole carbon source and energy source.During the course of the degradation of 3-HBA,gentisate was produced as an intermediate.Through spectrophotometric analyses on cell extracts of ZWL73 induced with 3-HBA,it was indicated that glutathione(GSH)-dependent gentisate pathway is involved in the 3-HBA catabolism in ZWL73 and the catabolism of this pathway must be induced with 3-HBA.更多还原