【摘要】 建立了毛细管气相色谱快速测定瘤胃发酵产物中甲烷和乙、丙、丁酸含量的方法.用HP-INNOWAX(19091N-133)毛细管柱,以高纯氮作为载气,用氢火焰离子化检测器对甲烷和乙、丙、丁酸含量进行测定.通过对总压力、总流量、柱流量、柱温、气化室和检测室温度等检测条件的对比,建立测定甲烷的理想检测条件为:总压力130 kPa、总流量30.2 mL?min-1,柱流量1.7 mL?min-1,线速度39.8 cm?s-1,柱温80℃,气化室温度100℃,检测室温度120℃;测定乙、丙、丁酸的理想检测条件为:总压力90 kPa、总流量37.2 mL?min-1,柱流量0.67 mL?min-1,线速度23.4 cm?s-1,柱温180℃,气化室温度200℃,检测室温度220℃.更多还原

【Abstract】 A rapid determination method of ruminal fermentation products by gas chromatogram has been developed. By using the HP-INNOWAX,FID and nitrogen as the carrier,the optimal condition for methane measurement was: nitrogen pressure at 130 kPa,total and column flow rates at 30.2 and（1.7 mL·）min^-1,and the column,injection and detection temperatures at 80,100 and 120 ℃,respectively.The optimal condition for acetate,propionate and butyrate measurement was: nitrogen pressure at（90 kPa,） total and column flow rates at 37.2 and 0.67 mL·min^-1,and column,injection and detection temperatures at 180,200 and 220 ℃,respectively.更多还原