【摘要】 建立了高效毛细管电泳测定猪尿中莱克多巴胺残留量的方法。通过对缓冲体系的种类、浓度、酸度、分离时间、分离电压及进样时间等条件对分离影响的研究,明确了电泳分析的最佳条件。在214 nm波长处,分离电压为15 kV,进样时间为20 s,分离时间为12 min,80 mmol/L的醋酸—醋酸钠(pH 5.04)运行缓冲液下,莱克多巴胺得到完全分离。莱克多巴胺质量浓度与电泳峰面积在0.05~100.00μg/mL范围内呈现良好的线性,相关系数为0.9961,最低检测限为0.05μg/mL,添加回收率为82.0%~94.3%。更多还原

【Abstract】 A method for determination of ractopamine residue in swine urine by high-performance capillary electrophoresis(HPCE) was set up.The effects of some important factors such as type,concentration and acidity of running buffer,separation time,separation voltage,and injection time were investigated to acquire the optimum condition.In 214 nm wavelength,ractopamine could be well separated within 12 min on a 57 cm length capillary at the separation voltage of 15 kV, the injection time of 20 s in 80 mmol/L acetic acid-NaAC buffer solution(pH 5.04).The calibration curve of ractopamine was linear by plotting the peak area against the sample concentration over the range from 0.05 μg/mL to 100 μg/mL.The correlative coefficiency was 0.9961.The recoveries of ractopamine were between 82.0% and 94.3% with a detecion limit of 0.05 μg/mL.更多还原