【摘要】 目的研究卡维地洛对心肌bcl-2、bax、TNF-α、TNF-αR1蛋白的影响。方法SD大鼠随机分四组：正常对照组,阿霉素(ADR)组,阿霉素+卡维地洛(ADR+CARV)组,卡维地洛(CARV)组。阿霉素腹腔注射(累计15mg/kg,分6次注射)制备心肌毒性动物模型；ADR+CARV组在注射阿霉素之后立即腹腔注射卡维地洛(累计10mg/kg,分6次注射)；卡维地洛组(累计10mg/kg,分6次注射)；等量生理盐水用于对照组。于第三周第七天处死大鼠,采用Western blot方法。结果TNF-α、bcl-2的检测：ADR组条带明显；ADR+CARV组条带不明显或已消失；正常对照组和CARV组无条带。bax与TNF-αR1的检测：在检测膜上目标位置均未出现明显条带。结论卡维地洛能够抑制TNF-α的表达,增强bcl-2的表达,但对bax和TNF-αR1的表达作用不明显。从蛋白水平上再度证明卡维地洛能够增强bcl-2的表达,但对bax的表达作用不明显,迟于分子生物学水平的表达。在蛋白水平上表明：卡维地洛能够抑制TNF-α的表达,但对TNF-αR1的表达作用不明显,不同于分子生物学水平的表达。更多还原

【Abstract】 Objective To study the protective effects of carvedilol on rats cardiomyocytes exposed to adriamycin.Methods SD rats were randomly divided into four groups:control group,adriamycin group,adriamycin+carvedilol group,carvedilol group.The models of myocardium toxicity were prepared with intraperitoneal injection of adriamycin(total 15 mg/kg,injection by 6 times);Carve- dilol was applied intraperitoneally followed adriamycin administration with a dose of(total 2 mg/kg,injection by 6 times);the equiva- lent volume of normal saline was used in control group.The heart tissues were obtained at the 21st day after the first time drug adminis- tration.The Western blot were performed to assess the expression alternation of bcl-2,bax,TNF-αand TNF-αR1 in protein levels. Results①The expression of TNF-αshowed clearly in adriamycin group and obscurly or dispeared in,adriamycin+carvedilol group, no sign in carvedilol group and control group.②The expression of bcl-2 showed clearly in carvedilol group and obscurly or dispeared in,adriamycin+carvedilol group,no sign in adriamycin group and control group.Conclusions Carvedilol may promote the expression of bcl-2 and inhibit the expression of TNF-α,but there is no clear influence to bax and TNF-αR1 by carvedilol.Tthese data may provide useful clues to elucidating the protective mechanism of carvedilol in protein levels.更多还原