【摘要】 目的探讨经导管冠状动脉内导入碱性成纤维细胞生长因子(bFGF)基因(pcDNA3-bFGF)对缩小猪急性心肌梗死面积的作用。方法实验动物分为两组:手术对照组(n=6),冠脉内bFGF基因导入组(n=6)。开胸结扎猪冠状动脉左旋支(LCX),建立急性心肌梗死动物模型。构建真核表达质粒pcDNA3-bFGF,并经导管冠脉内导入,通过心肌酶学及碱性复红苦味酸法(HBFP)特殊染色观察pcDNA3-bFGF对猪急性心肌梗死面积的影响。结果术后24 h,两组乳酸脱氢酶同工酶(LDH-1)及CK-MB较术前明显升高(P<0.01);术后48 h,bFGF基因导入组LDH-1及CK-MB明显低于手术对照组(P<0.01)。HBFP特殊染色显示,bFGF基因导入组心肌梗死面积明显小于手术对照组(P<0.05)。结论经导管冠状动脉内导入bFGF基因有缩小猪急性心肌梗死面积的作用。更多还原

【Abstract】 Objective To investigate the effects of basic fibroblast growth factor gene on the reduction of infarction size in a swine model of acute myocardial infarction.Methods All the swines were divided into two groups: operation control group(n=6) and intracoronary gene group(n=6).Swine models were made by opening chest and ligating the left circumflex(LCX) coronary artery.pcDNA3-bFGF eukaryotic expression plasmid was constructed and was directly injected into coronary artery by the catheter to observe the influences on infarction size according to myocardial enzyme and HBFP patho-histological sections.Results 24 hours(h) after operation,the values of LDH-1 and CK-MB were higher than that of before operation(P<0.01).48 h after operation,the values of LDH-1 and CK-MB in bFGF gene group were lower than those in control group(P<0.01).In bFGF gene group,infarction size was smaller than that in control group(P<0.05).Conclusions bFGF gene injected into coronary artery by the catheter can reduce infarction size in the swine models of acute myocardial infarction.更多还原