【摘要】 目的观察天螺霜体内、体外抗乙型肝炎病毒作用。方法采用实时荧光定量PCR(FQ-PCR)体外抑制试验及鸭乙型肝炎动物模型体内试验观察天螺霜对乙型肝炎病毒复制过程的影响。结果FQ-PCR试验显示,蒸馏水组与天螺霜组DNA拷贝中位数分别为6.9×108/ml与2.6×104/ml,差异有统计学意义(u=2.76,P<0.01)。体内试验25mg.kg-1.d-1和100mg.kg-1.d-1剂量组在用药5d、10d及停药3d时均能显著降低鸭乙肝病毒(DHBV)感染模型动物血清DHBV-DNA水平(P<0.05或P<0.01),两剂量组用药5d、10d及停药3d时DHBV-DNA抑制率分别为51.84%,55.32%,36.88%和81.08%,87.10%,61.80%。结论天螺霜体内、体外均具有抗乙型肝炎病毒复制的生物学作用。更多还原

【Abstract】 Objective To study the therapeutic effect of tianluo decoction against hepatitis B virus(HBV) in vivo and vitro.Methods The inhibitive effect were observed in the duplication process of hepatitis B virus by fluorescence quantitative PCR(FQ-PCR) in vitro test and duck’s hepatitis B animal model in vivo test.Results FQ-PCR in vitro test demonstrated that tianluo decoction had biological function to inhibit from duplication of hepatitis B virus,and there were significant difference in inhibit hepatitis B virus from duplicating in distilled water and tianluo decoction group(median:6.9×108/ml vs 2.6×104/ml,u=2.76,P<0.01).The test in vivo showed that tianluo decoction at the dosage of 25mg·kg -1 ·d -1 and 100mg·kg -1 ·d -1 had an obvious inhibitory effect on DHBV-DNA on the fifth and tenth day during treatment and the third day after treatment(P<0.05 or P<0.01).One group’s inhibitive rate of DHBV-DNA on the fifth and tenth day during treatment and the third day after treatment was 51.84%,55.32%,36.88%,respectively,and the other was 81.08%,87.10%,61.80% respectively.Conclusion Tianluo decoction has biologic function to inhibit from duplication of hepatitis B virus in vivo and vitro.更多还原