【摘要】 目的阐明山东济南地区婴幼儿腹泻中杯状病毒感染的流行状况及其特征。方法收集济南市儿童医院婴幼儿病毒性腹泻粪便标本,使用杯状病毒特异性引物对标本进行RT-PCR检测。将杯状病毒阳性PCR产物纯化后,进行克隆和序列分析,使用BioEdit和MEGA软件与GenBank中的参考株进行核苷酸序列同源性分析并绘制系统进化树。结果在212例病毒性腹泻标本中,杯状病毒RT-PCR阳性62例,检出率为29.25%。经序列分析,所有杯状病毒均为诺如病毒基因组Ⅱ型。结论杯状病毒是山东地区婴幼儿腹泻病的主要病原之一,目前以诺如病毒基因组Ⅱ型毒株流行为主。更多还原

【Abstract】 Objective To characterize the prevalence and genetics of human calicivirus (HuCV) infection in infants with acute diarrhea in Jinan area. Methods The fecal specimens from children with acute nonbacterial gastroenteritis were collected and HuCV in the samples were detected by reverse transcription (RT)-PCR. The amplified cDNA fragments were cloned into pMD18-T vector, sequenced and compared with the HuCV sequences deposited in GenBank. The nucleotide sequence homology was evaluated with BioEdit software, and the phylogenetic tree was generated by MEGA 3.0. Results HuCV was detectable in 62 cases out of the 212 specimens (29.25%). Nucleotide sequence analysis revealed that the virus strains all belonged to Norovirus Genogroup II. Conclusion HuCV is one of the most important pathogens causing diarrhea in infants and young children in Jinan area. Norovirus Genogroup II is the major HuCV agent.更多还原