【摘要】 目的研究结缔组织生长因子(connective tissue growth factor,CTGF)与转化生长因子-β1(transforminggrowth factor-beta1,TGF-β1)在日本血吸虫病小鼠肝纤维化肝脏中的表达状况及意义。方法用昆明小鼠感染尾蚴建立日本血吸虫病小鼠肝纤维化模型,随机分为模型组和对照组,感染后6、10、14、18周杀鼠;HE染色观察肝脏病理变化,免疫组化法检测肝内CTGF、TGF-β1蛋白的表达水平。结果模型组小鼠10周时形成血吸虫病肝纤维化,此时肝内CTGF表达达高峰,且10、14、18周时的表达量均显著高于同期对照组(P<0.01);模型组TGF-β1蛋白定量和CT-GF有相同的变化趋势,但18周时与同期对照组比较差异无显著性(P>0.05);模型组CTGF和TGF-β1蛋白表达水平具有直线相关性。结论CTGF与TGF-β1的表达与日本血吸虫病小鼠肝纤维化形成有密切关系,TGF-β1的致纤维化作用可能部分通过CTGF的生物学作用介导,阻断CTGF的传导通路可能是血吸虫病肝纤维化治疗的有效靶点。更多还原

【Abstract】 Objective To investigate the hepatic expression of connective tissue growth factor（CTGF） and transforming growth factor-beta1（TGF-β₁） in murine schistosomiasis liver fibrosis. Methods The mice infected with Schistosomas japonicum were randomly divided into the model group and the control group,and subsequently killed at the 6th,10th,14th,18th weeks respectively.The hepatic expression of CTGF and TGF-β₁ was detected by immunohistochemical method.Histological changes in HE staining were observed by light microscopy.Results The models of murine schistosomiasis liver fibrosis were successfully established at the 10th week.The expression peak of CTGF of medal groups showed at the 10th week and it had remarked difference compared with that of the control groups at the 10th,the 14th,the 18th week,respectively.The expression of TGF-β₁ had the same tendency with CTGF,but it had no marked difference at the 18th week from that in the control group.The hepatic expression of CTGF and TGF-β₁ in the model groups had positive correlation.Conclusion The expression of CTGF and TGF-β₁ is closely correlated to the murine schistosomiasis liver fibrosis and TGF-β₁ may play its roles mediated by CTGF in liver fibrogenesis.CTGF may be a potential target to block the development of schistosomiasis liver fibrosis.更多还原