【摘要】 目的克隆土耳其斯坦东毕吸虫磷酸丙糖异构酶的全长基因。方法根据日本血吸虫和曼氏血吸虫磷酸丙糖异构酶基因的保守区设计引物,利用RT-PCR扩增出土耳其斯坦东毕吸虫磷酸丙糖异构酶基因的大片段,再结合RACE技术分别得到磷酸丙糖异构酶基因的3′端和5′端,将3部分序列拼接后获得磷酸丙糖异构酶基因全长cDNA序列,并提交GenBank。结果成功克隆了土耳其斯坦东毕吸虫磷酸丙糖异构酶基因全长cDNA序列并提交GenBank,登录号为DQ092331。结论土耳其斯坦东毕吸虫磷酸丙糖异构酶基因全长cDNA的克隆为进一步表达及其生物学性能的分析提供了理论基础。更多还原

【Abstract】 Objective To clone full-length triosephosphate isomerase gene of Orientobilhazia turkestanicum.Methods The partial encoding region of triosephosphate isomerase gene was cloned using RT-PCR.The 3′end fragment and 5’end fragment of triosephosphate isomerase gene were obtained by 3′RACE and 5′RACE.The full-length cDNA sequence was gotten by splicing sequences of the three fragments.The cDNA sequence was submitted to GeneBank.Results The full-length cDNA sequence of triosephosphate isomerase gene was successfully cloned and its GeneBank accession number is DQ092331.Conclusion The cloning of triosephosphate isomeras gene lays the theoretical foundation for the further expression and biological analysis of triosephosphate isomeras gene full-length cDNA sequence.更多还原