【摘要】 目的建立瘢痕疙瘩家系永生细胞库,为瘢痕疙瘩发病机制的研究提供永久的研究材料。方法采用EB病毒(Epstein-Barrvirus)转化技术,把瘢痕疙瘩家系外周血B淋巴细胞转化成永生化淋巴母细胞系(LCL)。结果成功地为一个瘢痕疙瘩家系27个个体建立了永生细胞株,所有的细胞株冻存后复苏的成功率为100%。结论瘢痕疙瘩家系永生细胞库保存了原来个体完整的基因组,为以后的深入研究提供用之不竭的DNA资源。更多还原

【Abstract】 Objective To provide perpetual research materials for long term studies by establishing immortal lymphoblastoid cell bank of keloids pedigree. Methods The immortal lymphoblastoid cell lines of keloids pedigree were established by Epstein-Barr virus transformation of peripheral blood B lymphocytes. Results 27 immortal lymphoblastoid cell lines of keloids pedigree were obtained successfully, all of the immortal lymphoblastoid cell lines were successfully revivificated after been frozen in liquid nitrogen. Conclusions It is important to establish immortal lymphoblastoid cell bank of keloids pedigree and provide long-term DNA materials for deep study of keloids in the future.更多还原