【摘要】 目的探讨乳腺癌患者骨髓中人乳腺珠蛋白(hMAM)mRNA的表达及其临床意义。方法应用巢式RT-PCR技术,同时检测75例乳腺癌患者、15例乳腺良性病变患者和8例健康人骨髓中hMAM mRNA的表达,分析hMAM mRNA表达与临床病理因素、Ki67、p53和血管内皮生长因子(VEGF)的关系。结果RT-PCR检测的敏感度达到10-6。75例乳腺癌患者中,21例检测出hMAM mRNA阳性表达,阳性表达率为28．0％。hMAM mRNA阳性表达与乳腺癌腋窝淋巴结转移和PR状况有关(P<0．05),与年龄、肿瘤大小、临床分期和ER状况无关(P>0．05);与乳腺癌组织Ki67表达呈正相关(x2=4．936,P=0．026)。乳腺良性病变患者和健康人骨髓中,未检测到hMAM mRNA表达。结论应用RT-PCR方法检测乳腺癌骨髓中hMAM mRNA的表达,敏感度高、特异性好。hMAM mRNA可作为检测乳腺癌患者骨髓中播散肿瘤细胞的分子指标之一,可为乳腺癌患者的治疗和预后判断提供帮助。更多还原

【Abstract】 Objective To investigate the expression of the human mammoglobin (hMAM) mRNA in bone marrow and its clinical significance in the breast cancer patient. Methods Expression of hMAM mRNA was detected using nested reverse transcription polymerase chain reaction (RT-PCR) in the bone marrow aspiration sample from 75 breast cancer patients, 15 patients with benign breast lesions and 8 healthy volunteers as control. The possible correlation of hMAM mRNA expression with clinico-pathological parameters and related molecular markers such as Ki67, p53 and VEGF were analyzed. Results The sensitivity of RT-PCR in this series reached 10 -6. The hMAM mRNA was found to be positively expressed by RT-PCR in 21 of 75 breast cancer patients with a positive rate of 28. 0%. However, hMAM mRNA expression was not detected in the bone marrow aspiration samples from patients with benign breast lesions and healthy volunteers. The hMAM mRNA expression was positively correlated with axillary nodal involvement and progesterone receptor (PR) status (P<0. 05) as well as Ki67 expression in breast cancer tissue (x2 =4.936, P = 0.026) , but not with age, tumor size, clinical stage, or estrogen receptor (ER) status (P>0. 05). Conclusion RT-PCR is quite sensitive and has a high specificity in detecting the presence of hMAM mRNA in the bone marrow from breast cancer patients. Thereupon, hMAM mRNA may be useful as a molecular biomarker in detecting disseminated tumor cells( DTC) in the bone marrow of breast cancer patients. Positive hMAM mRNA expression result may have an impact upon therapeutic recommendations and patients’ prognostic judgement.更多还原