【摘要】 目的了解慢性淋巴细胞白血病(CLL)的分子遗传学特性。方法运用间期荧光原位杂交(FISH)技术对60例初发的B细胞CLL(B-CLL)患者进行12号染色体3体(+12)、del (13q14)和del(17p13)检测。结果 60例患者中,41例(68．3％)至少有一种分子遗传学异常, 2例(3．3％)具有2种染色体异常。12例(20．0％)有+12异常,其畸变细胞率在4．0％-34．0％之间;24例(40．0％)有del(13q14)异常,其畸变细胞率在22．0％-93．0％之间,其中3例有2条染色体del(13q14)异常;7例(11．7％)有del(17p13)异常,其畸变细胞率在6．0％-68．0％之间。不同Binet分期中,3种分子遗传学异常差异无统计学意义。结论FISH是一种在分析CLL染色体数目和结构异常方面较为快速、准确和敏感的方法,可为CLL的研究提供较为准确的分子遗传学信息。更多还原

【Abstract】 Objective To explore the molecular cytogenetic characteristics in patients with chronic lymphocytic leukemia (CLL). Methods Interphase fluorescence in situ hybridization (FISH) was used to detect trisomy 12, deletion of 13ql4 and 17pl3 in 60 patients with CLL. Results Out of the 60 patients, 41 (68. 3% ) had at least one kind of molecular cytogenetic aberrations. Two (3. 3% ) had two kinds of abnormalities. Trisomy 12 was found in 12 (20. 0%) cases, 13ql4 deletion in 24 (40. 0%) cases and 17pl3 deletion in 5 (11.7%) cases. The number of trisomy 12 cells ranged from 4.0% to 34.0% , 13ql4 deletion ranged from 22. 0% to 93. 0% and 17pl3 deletion ranged from 6. 0% to 68. 0%. There was no significant difference among each Binet stages. Conclusion FISH is a more rapid, accurate and sensitive technique in analysis of chromosome aberrations in CLL. FISH may provide accurate information of molecular cytogenetics for CLL.更多还原