【摘要】 目的探讨端粒酶hTERTmRNA表达在人乳腺癌发生、发展中的意义,观察肿瘤抑制基因p53与hTERTmRNA表达的关系。方法收集浸润性导管癌标本25例,导管原位癌标本18例,导管上皮不典型增生标本20例,导管上皮单纯性增生标本7例,癌旁正常乳腺组织标本12例。用原位杂交法检测hTERTmRNA表达,并用免疫组化方法检测乳腺导管癌的p53蛋白表达。结果hTERTmRNA在癌旁正常乳腺组织、乳腺导管单纯性增生中未见表达;在导管不典型增生、导管原位癌、浸润性导管癌中的阳性率分别为25.0%、83.3%和88.0%。导管原位癌、浸润性导管癌组织hTERTmRNA表达明显高于癌旁正常乳腺组织、乳腺导管单纯性增生和导管不典型增生组织(P<0.05)。hTERTmRNA表达与浸润性导管癌肿块大小及淋巴结转移与否无关(P>0.05)。43例乳腺导管癌中,hTERTmRNA表达与p53蛋白表达呈正相关(r=0.5540,P<0.01)。结论端粒酶hTERT mRNA表达可能在乳腺导管癌的组织发生中起关键作用,半定量原位检测hTERTmRNA表达,可为导管上皮不典型增生与导管原位癌的鉴别诊断提供帮助。p53突变可能与乳腺导管癌hTERT基因转录激活有关。更多还原

【Abstract】 Objective This study was designed to investigate the significance of hTERT mRNA in breast carcinogenesis and to explore the diagnostic efficacy, and to study the effect of tumor suppressor gene p53 on the expression of hTERT mRNA. Methods The expression of hTERT mRNA was examined by in situ hybridization in 12 cases of normal breast tissue nearby cancer, 7 of simple ductal hyperplasia, 20 of atypical hyperplasia, 18 of ductal carcinoma in situ and 25 with invasive ductal carcinoma. The expression of p53 protein were examined by immunohistochemistry in 43 carcinomas. Results hTERT was not detected in normal breast tissue nearby cancer and simple ductal hyperplasia. The positive rate of hTERT mRNA in atypical hyperplasia, ductal carcinoma in situ and invasive ductal carcinoma were 25.0%, 83.3% and 88.0%, respectively. The prevalence and intensity of hTERT mRNA expression were much greater in carcinoma than those in simple or atypical hyperplasia and normal breast tissue nearby cancer (P<0.05). The expression of hTERT was not correlated with tumor size and lymph node metastasis (P>0.05). The positive correlation between hTERT mRNA and p53 was found in breast carcinoma (r= 0.5540,P<0.01). Conclusion hTERT mRNA expression is closely related to the malignant transformation of breast tissue. Semi-quantitative detection of hTERT mRNA expression in situ is helpful in differentiated diagnosis of carcinoma in situ and atypical hyperplasia. Inactivation of p53 may play a role in the transcriptive activation of hTERT gene in breast carcinoma.更多还原