【摘要】 通过对珐菲亚进行离体培养快繁技术的研究,解决珐菲亚引种栽培中种源短缺问题。方法:用茎尖为外植体,以MS为基本培养基,附加不同浓度的激素进行培养。结果:用MS+BA 1.5 mg/L+NAA 0.5 mg/L诱导培养,40 d可获得丛生芽;继代用MS+BA 1.2 mg/L+NAA 0.2 mg/L培养,增殖率3.9;用1/2 MS+NAA 1.0 mg/L培养15 d可获得生根植株。结论:本研究得出的方法可为引种栽培珐菲亚提供大量种苗。更多还原

【Abstract】 Objective: To solve the shortage of Pfaffia paniculata seed resourse for introduction and cultivation by quick-propagation in vitro culture.Methods: The stem apex of Pfaffia paniculata was used as explants and cultivated in the minimal medium MS with different concentration of hormone.Results: The cluster buds were obtained after forty-day induction culture in the medium of MS+BA1.5mg/L+NAA0.5mg/L.The subculture medium was MS+BA1.2mg/L+NAA0.2mg/L.The rate of multiplication was 3.9.The medium(1/2MS)+NAA1.0mg/L was the best one for root inducement.Rooting plants were obtained after fifteen-day root induction culture.Conslusion: The quick-propagation in vitro culture could be used for providing the seedling of Pfaffia paniculata for the introduction and cultivation in large scale.更多还原