【摘要】 利用RAPD技术,对白粉病免疫的贵农21号与加拿大小麦Neepawa的BC1F1代100个抗感单株进行了分子标记辅助选择。利用随机引物S 2018进行RAPD分析结果表明,在抗病亲本贵农21号和回交F1代抗病单株中均扩增出特异的DNA片段,感病对照不能扩增出此带,该特异片段的分子长度约900 bp,且重复性好,稳定性好,说明该标记与贵农21的抗白粉病基因相连锁,平均选择符合率达91.86%,在其杂交后代中进行早代选择是有效的,为选育抗病小麦种质提供了有效的手段。更多还原

【Abstract】 Using RAPD technology,100 resistant and susceptible individual plants of BC₁F₁between Guinong 21 and Neepawa had been carried on the molecule marker-assisted selection.The RAPD amplification results with primer S2018 indicated,the DNA fragment were amplified in the resistant parent Guinong 21 and resistant materials of BC₁F₁ by the primer,the susceptible contrast could not amplify this fragment,and the DNA fragment amplified by the primer S2018 was about 900bp,then repetitions and stability were good.This experiment explained that this marker was linked with the resisted powdery mildew gene of Guinong 21,reaching 91.86% in selection rate,and it was an efficient selection in its filial generation,and it provided the effective method for the seed selection in resistant wheat.更多还原