【摘要】 目的了解耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)抗菌药物及消毒剂耐药基因流行病学状况。方法对40株MRCNS菌进行mecA、aac(6′)/aph(2″)、aph(3′)-Ⅲ、ermA/B/C、tetM、qacA/B检测。结果40株MRCNS菌mecA、aac(6′)/aph(2″)、aph(3′)-Ⅲ、ermA/B/C、tetM、qacA/B基因阳性株分别为39、32、15、30、2、6株,阳性率分别为97.5%、80.0%、37.5%、75.0%、5.0%、15.0%;其中同时检出mecA基因、aac(6′)/aph(2″)/和(或)aph(3′)-Ⅲ基因、ermA/B/C基因26株(65.0%)。结论65.0%MRCNS菌已同时携带mecA基因、aac(6′)/aph(2″)/或aph(3′)-Ⅲ基因、ermA/B/C基因。更多还原

【Abstract】 OBJECTIVE To study the epidemiology of drug-resistant and antiseptic-resistant genes of(productive)(enzyme) in meticillin-resistant coagulase negative Staphylococcus(MRCNS).METHODS mecA Gene of the(drug-)resistant β-lactam,aac(6′)/aph(2″) and aph(3′)-Ⅲ genes of aminoglycoside-modifying enzyme(AME) and(ermA/B/C) genes of erythromycin methyltransferase were detected by polymerase chain reaction(PCR) in 40 MRCNS strains.RESULTS From them there were 39 strains with mecA gene,32 strains with aac(6′)/aph(2″) gene,15 strains with aph(3′) -Ⅲ gene,30 strains with ermA/B/C gene,2 strains with tetM and 6 strains with qacA/B gene.There were 26 strains(65.0%) simultaneously with mecA,aac(6′)/aph(2″)and(or) aph(3′)-Ⅲ and ermA/B/C genes in MRCNS.CONCLUSIONS The drug-resistant rate is higher in MRCNS.There are more than half tested strains simultaneously with 3 to 4 drug-resistant genes.更多还原