【摘要】 目的探讨中心体α-微管蛋白和γ-微管蛋白在人乳腺癌前病变和乳腺癌中的表达及其在乳腺癌发生发展过程中的作用。方法采用免疫组织化学S-P法检测乳腺导管上皮不典型增生、乳腺导管内癌和浸润性导管癌各40例中中心体α-微管蛋白和γ-微管蛋白表达水平,30例正常乳腺组织作为对照组,并用W estern印迹进行验证。所得数据经统计学分析并结合随访情况进行评价。结果不同样本组α-微管蛋白、γ-微管蛋白表达水平不同(P=0.000),随正常上皮组织到出现不典型增生至进展为原位癌和浸润性癌的发展变化,α-微管蛋白、γ-微管蛋白表达量呈增高趋势,以浸润性癌为最高(40例中分别有14例、11例表达为+++),各组间差异均有统计学意义(P=0.001)。比较同例和同组α、γ-微管蛋白的表达程度差异无统计学意义(P>0.05)。癌前病变、导管内癌、浸润性导管癌组α、γ-微管蛋白的表达水平与预后具有相关性。结论中心体异常为乳腺癌细胞恶性表型的明显特征之一,并可能为乳腺癌形成过程中的早期事件。α、γ-微管蛋白的表达情况可为乳腺癌发生机制探讨、高危病例筛选及估测预后提供一种新的途径。更多还原

【Abstract】 Objective To investigate the expression of α-tubulin and γ-tubulin,2 kinds of centrosome proteins,in premalignant lesion and carcinoma of breast and the significance thereof.Methods(Forty specimens) of premalignant lesions of breast,40 specimens of infiltrating ductal carcinoma of breast,40 specimens of intraductal carcinoma(IDC),and 30 specimens of normal breast tissues were obtained during operation.Immunohistochemistry was used to analyze the protein expression of α-tubulin and γ-tubulin,and the percentages of ki67 positive cells.Western blotting was used to examine the mRNA expression of α-tubulin and γ-tubulin.Results The protein and mRNA expression values of α-tubulin and γ-tubulin in breast carcinoma were higher than those in the premalignant lesions and normal breast tissues with significant differences between the premalignant lesions and normal breast toques and without significant differences between infiltrating ductal carcinoma of breast and IDC.The ki67 positive rates of the infiltrating ductal carcinoma of breast group,IDC group,premalignant lesion group,and normal breast tissues group were 16.0%,37.0%,53.6%,and 67.8%(P=0.001).A positive correlation existed between the expression of α-tubulin and the expression of γ-tubulin in the same case and the same group(all P=0.00) and there was no significant correlation between the expression of α-tubulin and the expression of γ-tubulin in the same case and the same group(all P>0.05).Both the expression of α-tubulin and the expression of γ-tubulin were significantly associated with the prognosis.Conclusions Centrosome protein is one of the distinct phenotypes of breast cancer cells.Aberration of centrosome proteins may be used to screen high risk cases of breast carcinoma and to estimate the prognosis.更多还原