【摘要】 目的探讨晶状体后囊膜上晶状体上皮细胞的增生分化规律和普拉洛芬滴眼液对晶状体后囊膜混浊(PCO)的抑制作用。方法将牛后囊膜晶状体上皮细胞面朝上平铺于25ml培养瓶底部,分别加入含0%、10%、20%胎牛血清的DMEM培养液培养1~5周,计数后囊膜晶状体上皮细胞覆盖率,并用Giemsa染色和扫描电镜观察其形态。同时用相当于普拉洛芬滴眼液滴眼后4h在房水中的浓度(0.23mg/L)作用于组织培养模型,与对照组比较,观察其对后囊膜晶状体上皮细胞融合时间的影响。结果赤道部的晶状体上皮细胞向晶状体后囊膜增生、迁徙,晶状体后囊膜形成明显的混浊和皱缩;后囊膜晶状体上皮细胞融合天数随血清浓度的升高而缩短;浓度为0.23mg/L的普拉洛芬对后囊膜晶状体上皮细胞的融合有明显抑制作用。讨论体外模拟PCO的组织培养是研究后发性白内障的有效方法。普拉洛芬是一种安全、有效降低PCO发生率的药物,可作为白内障术后的常规用药。更多还原

【Abstract】 Objective To establish an in vitro model of posterior capsule opacification (PCO) by culturing the posterior capsule of bovine lens, to observe the proliferation and differentiation of lens epithelial cells and to study the influence of serum and pranoprofen eyedrops on cell confluence of this model. Methods The bovine lens posterior capsule was spread on the surface of a 25 ml culture flask with cell layer upward. DMEM with 0%, 10% and 20% fetal calf serum was used as culture medium. The cell coverage and confluence time on the posterior capsule were observed by inverted microscope and the cell morphology was observed by Giemsa staining and scanning electron-microscope. Pranoprofen was added to the culture medium at a concentration similar to the aqueous humor concentration (0.23 mg/L), which was presented at 4 hours after the instillation of pranoprofen eyedrops. The difference of confluence time between the treated group and the control group was compared. Results The lens epithelial cells migrated and proliferated rapidly on the posterior capsule from the equatorial region to the center. The cell coverage was increased and the confluence time was shortened with the increase of serum concentration (P<0.05). The PCO and wrinkles were presented. Pranoprofen at 0.23 mg/L could inhibit the confluence of lens epithelial cells (P<0.01). Conclusions The in vitro model for PCO was an useful method to study the mechanism of PCO formation. Pranoprofen can inhibit the proliferation of lens epithelial cells and is a safe and efficient drug for preventing the occurrence of PCO and can be used as a routine medication after the cataract operation.更多还原