【摘要】 目的探讨测定血浆壳三糖苷酶活性的荧光分光光度法,同时评估方法的可靠性。方法采用荧光分光光度法测定了44例10~14岁的健康儿童血浆壳三糖苷酶的活性,同时对实验条件、方法的检出限和精密度等多方面进行了分析。结果本法的最佳激发波长和发射波长分别为358nm和458nm。最低检出限为3.24nmol.h-1.ml-1。回归方程为Y=38.124X+111.6,r=0.997、P<0.01。该方法得出的样品日内和日间CV值分别6.43%和12.70%。44例10~14岁男性和女性儿童该酶活性的数值分别为(11.69±1.71)nmol.h-1.ml-1和(11.07±1.28)nmol.h-1.ml-1。结论血浆壳三糖苷酶的活性是临床溶酶体疾病的筛查的一个重要生物学指标,初步应用结果表明,荧光分光光度法是分析该酶活性的一种简单、快速、灵敏、可靠的方法。更多还原

【Abstract】 Objective To determine the plasma chitotriosidase activity by use of fluorescence spectrophotometry and evaluate the reliability of the method.Methods The plasma chitotriosidase activity in 44 healthy children were measured by F-4500 fluorescence spectrophotometer and the experimental condition, the detection limit and the precision etc were analyzed.Results The best λex and λem was 358 nm and 448 nm respectively.The detection limit of the method was 3.24 nmol·h -1 ·ml -1 .The regression equation was Y=38.124X+111.6 and the correlation coefficient(r) was 0.997(P<0.01).The intra-day and inter-day variation for plasma chitotriosidase activity was 6.43% and 12.70% respectively.The values of the plasma chitotriosidase activity in these 44 healthy boys and girls aged 10-14 were (11.69±1.71) and (1.07±1.28)nmol·h -1 ·ml -1 .Conclusions The plasma chitotriosidase activity is an important biochemical marker for the screen of lysosomal storage diseases.The preliminary clinical application results showed that the fluorescence spectrophotometry method was a simple, rapid, sensitive and reliable analytical method for determination of this enzyme.更多还原