【摘要】 目的应用多重聚合酶链反应(PCR)对临床分离的耐甲氧西林金黄色葡萄球菌的SCCmec基因型及亚型进行分型。方法收集本院2005年2月至7月临床分离的112株金黄色葡萄球菌,应用PCR扩增mecA基因检测MRSA,用多重PCR检测MRSA的SCCmec的基因型及亚型。用全自动微生物分析仪进行药敏试验。结果72株金黄色葡萄球菌的mecA基因阳性,确定为MRSA,阳性率为64·3%(72/112)。SCCmecⅢ和SCCmecⅢA的阳性率分别为68·1%(49/72)和20·8%(15/72),H16和H56为SCCmecⅡ,H19为SCCmecⅣ,5株MRSA没有被分型。携带SCCmecⅡ、SCCmecⅢ或SCCmecⅢA的菌株均为多重耐药株,而携带SCCmecⅣ的菌株除对β内酰胺类药物耐药外,对其他类别的抗菌素敏感。结论本院分离的MRSA以SCCmecⅢ和SCCmecⅢA为主。多重PCR对MRSA进行SCCmec基因分型结果可靠,适合临床实验室。携带SCCmecⅡ、SCCmecⅢ或SCCmecⅢA的临床分离株耐药性严重。更多还原

【Abstract】 Objective To identify the genotypes of Staphylococcal chromosomal cassette mec(SCCmec) in the isolates of methicillin-resistant Staphylococcus aureus(MRSA) by multiplex polymerase chain reaction(PCR). Methods 112 isolates of Staphylococcus aureus were isolated from various clinical specimens from Feb.2005 to Jul.2005 in our hospital. PCR was used for detecting mecA gene in Staphylococcus aureus. The genotypes and subgenotypes of SCCmec were determined by multiplex PCR. The antimicrobial susceptiblity test was proceeded by automated Microbiology Analyzer. Results 72 of 112(64.3%) Staphylococcus aureus were MRSA with mecA gene positive. The positive rates of SCCmecⅢ and SCCmecⅢA were 68.1%(49/72) and 20.8%(15/72). H16 and H56 were SCCmecⅡ. Only one isolate was SCCmecⅣ. 5 MRSA strains were non-typeable. The isolates with SCCmecⅡ、SCCmecⅢ or SCCmecⅢA were multi-resistant to antibiotics.H19 with SCCmecⅣ was susceptible to all antibiotics except β lactams. Conclusion SCCmecⅢ and SCCmecⅢA are major genotypes in MRSA in Wenzhou area, China. Multiplex PCR is rapid, robust and capable of identifying the genotypes and subgenotypes of SCCmec in MRSA. The isolates with SCCmecⅡ, SCCmecⅢ or SCCmecⅢA are multi-resistant to antibiotics.更多还原