【摘要】 目的探讨肝癌p33ING1bmRNA表达水平及与临床病理特征的关系,揭示p33ING1b基因在肝癌发生发展中的作用及临床诊断意义。方法用逆转录聚合酶链反应(RT-PCR)检测55例原发性肝癌患者癌组织及其癌旁组织p33ING1bmRNA水平。结果63·6%(35/55)癌组织及70·9%(39/55)癌旁组织p33ING1bmRNA呈阳性表达,癌组织p33ING1bmRNA表达量为0·410±0·175,明显低于癌旁组织0·529±0·203,两者比较差异有统计学意义(P<0·05)。p33ING1bmRNA水平与肝癌的病理分级、淋巴结转移、包膜浸润与否有关,而与年龄、性别、肿瘤大小、癌栓形成、合并肝硬化与否无关。结论p33ING1b表达下调在肝癌发生、发展中起重要作用,可以作为肝癌分化、侵袭性及预后的评价指标。更多还原

【Abstract】 Objective To investigate the p33~ ING1b mRNA level and the relationship to clinical pathological data in primary hepatocellular carcinoma(PHC).Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) method was employed to detect the mRNA level of p33~ ING1b in liver cancer tissues and adjacent tissues of cancer.Results p33~ ING1b mRNA was positive expression in 63.6% (35/55) cancer tissues and 70.9% (39/55) adjacent tissues of cancer.The expression level of p33~ ING1b mRNA in cancer tissues (0.410±0.175) was significantly lower than that in adjacent tissues of cancer (0.529±0.203). The level of p33~ ING1b was significantly associated with pathological grades, lymph node metastasis and envelope infiltration, but not associated with sex, age, tumor size, tumor thrombus or liver cirrhosis.Conclusion The decrease of p33~ ING1b may play an important role in the tumorigenesis and development of PHC, and may be an adjuvant parameter in evaluating tumor differentiation, invasion and prognosis.更多还原