节点文献

淋巴系统恶性肿瘤患者血清游离DNA含量与重排基因检测及其临床意义

The research of serum-DNA IgH and T-cell receptor gama gene rearrangement in lymphogenous malignant patients

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 晏开力任维华徐修才翟志敏

【Author】 YAN Kai-li, REN Wei-hua, XU Xiu-cai, ZHAI Zhi-min.Department of Central Experimental Laboratory, Anhui Provincial Hospital, Hefei 230000, China

【机构】 安徽省立医院中心实验室安徽省立医院中心实验室

【摘要】 目的探讨血清游离DNA含量及其克隆性重排基因检测在淋巴系统恶性肿瘤诊断中的价值。方法常规方法提取与定量分析72例淋巴系统恶性肿瘤患者血清DNA;用聚合酶链反应分别对不同患者血清单个核细胞(PBMC)DNA的特异性免疫球蛋白重链CDRⅢ区、T淋巴细胞受体γV9区序列进行扩增、检测和对照分析。结果急性淋巴细胞白血病(ALL,含B-ALL组和T-ALL组)、B-淋巴瘤组、T-淋巴瘤组患者血清游离DNA含量[分别为(418±172)μg/ml、(426±192)μg/ml、(388±170)μg/ml、(400±110)μg/ml]均高于正常对照组[(77±47)μg/ml,P<0·05]。其中46例ALL患者血清及PBMC重排基因阳性率分别为76·1%和82·6%;26例淋巴瘤患者血清及PBMC重排基因阳性率分别为73·1%和65·4%;且两种方法检测结果有较好的一致性(P>0·05)。结论淋巴系统恶性肿瘤患者血清游离DNA含量明显增高,且易检测出特征性重排基因,血清游离DNA和重排基因对淋巴系统肿瘤诊断有重要价值。

【Abstract】 Objective To evaluate the diagnostic significance of detecting serum-DNA concentration and clonal gene rearrangement in lymphogenous malignant patients.Methods Serum-DNA in 72 diagnosed patients were measured and analyzed by SPSS11.0.Serum and mono-nuclear cells samples were collected. IgH CDRⅢ, TCRγV9 region were amplified by PCR.Results The serum-DNA concentration of disease groups were (418±172)μg/ml,(426±192)μg/ml, (388±170)μg/ml and(400±110)μg/ml, each was higher than those of control group(77±47)μg/ml(P<0.05).Among the 46 ALL cases, the gene rearrangement positive ratio of serum and mononuclear cells samples were 76.1%、82.6% respectively. Among the 26 lymphoma cases, the positive ratio of serum and mononuclear cells samples were 73.1%、65.4% respectively. Analyzed by SPSS11.0, the positive ratio of serum sample was highly consistent with mononuclear cells sample in lymphogenous malignant patients(P>0.05).Conclusion The concentration of serum-DNAwere higher in lymphogenous malignant patients and tumor associated DNA could be easily detected, so they have important diagnostic value in lymphogenous malignant patients.

【基金】 安徽省优秀青年科技基金资助项目(20020318)
  • 【文献出处】 中华检验医学杂志 ,Chinese Journal of Laboratory Medicine , 编辑部邮箱 ,2006年10期
  • 【分类号】R733
  • 【被引频次】1
  • 【下载频次】180
节点文献中: 

本文链接的文献网络图示:

本文的引文网络