【摘要】 目的了解浙江省沿海地区的海及水产品霍乱弧菌污染状况和菌型特征,为制定海及水产品卫生管理措施和霍乱防治对策提供科学依据。方法选择3个沿海县市,分别在农贸市场、养殖场(或捕捞点)、餐馆等场所采集990份海及水产品标本,标本经碱性蛋白胨水增菌,4号培养基选择性培养,生化鉴定和血清、噬菌体分型,毒力基因检测。结果海及水产品带菌率为1.41%,其中甲鱼带菌率最高为8.9%。有稻叶型、小川型、0139群等3个血清型,各型菌株数分别为2株、2株、10株。12株菌株中9株有毒力基因,其中5株有3个毒力基因,1株只有 CTX、ACE 毒力基因,3株只有 ZOT 毒力基因。结论浙江省沿海地区海及水产品受到霍乱弧菌污染,霍乱弧菌3种菌型全有,大多具有毒力基因,能引起霍乱暴发的危险。更多还原

【Abstract】 Objective To examine vibrio cholera (V.C) in aquatic products of littoral erea, Zhejiang Province and to provide scientific evidence for administration of aquatic products and cholera epidemic control.Methods All 990 samples of aquatic products collected from local markets,eateries and aquafarms in three chosed areas.Samples were proliferated in alkaline liquid medium,and purified in NO :4 medium,the isolations were identified biochemically,and phenotype of strains were defined by phagocyte and coagulation with V.C.diagnostic serum.Three virulence genes (ctx,ace,zct ) of the isolated strains were detected by polymerase chain reaction (PCR).Results There were 1.41% samples caught by V.C., having a carrying rate highest in turtles of 8.9%.14 strains were defined as three serogroups,and the numbers of Inaba,Ogawa,and Hikojima types were 2,2,10 respectively.Virulence genes had detected in 9 of 12 stains.All genes were detected in 5 strains ,only ZOT genes in 3 strains,and both CTX and ACE genes in 1 strain.Conclusion Aquatic products from inshore in Zhejiang Province caught with V.C. strains might be divided into three serogroups.Most of them should be virulence genes.Cholera epidemic outbreak might be caused by those contaminated products.更多还原