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应用荧光原位杂交技术检测卵巢浆液性囊腺癌中ZNF217基因拷贝数
ZNF217 gene was detected in ovarian serous cystadenocarcinoma by fluorescence in situ hybridization
【摘要】 目的探讨锌指蛋白217(zincfingerprotein217,ZNF217)基因在卵巢浆液性囊腺癌中20号染色体基因拷贝数改变情况及其临床意义。方法应用荧光原位杂交技术及LSIZNF217探针对2种卵巢癌细胞株SKOV3、HO-8910和23例卵巢浆液性囊腺癌、10例浆液性囊腺瘤及7份正常卵巢组织进行检测。结果两种卵巢癌细胞株及12例卵巢癌出现ZNF217基因扩增,浆液性囊腺瘤有1例发生了基因的扩增,其余的及正常卵巢组织未出现基因扩增,卵巢癌与卵巢浆液性囊腺瘤、正常卵巢细胞相比较,ZNF217基因拷贝数改变差异有统计学意义(P<0.05),而低分化的卵巢癌比高分化发生ZNF217基因扩增的几率明显增高(P<0.05),Ⅲ~Ⅳ期比Ⅰ期的卵巢癌ZNF217基因拷贝数明显增多(P<0.05)。结论ZNF217基因很可能是卵巢癌发生的促进因子之一,并与卵巢癌分化及预后不良有关。
【Abstract】 Objective To investigate amplification of zinc finger protein 217(?ZNF217)gene in ovarian serous cystadenocarcinoma and its clinical significance.Methods Twenty three specimens of ovarian carcinoma,10 specimens of ovarian benign tumors and 7 specimens of normal ovaries and two ovarian cancer cell lines,SKOV3 and HO-8910 were examined by fluorescence in situ hybridization(FISH).Results The amplification of ?ZNF217 was gained in 12 case of ovarian cancers,there was only 1 case in ovarian benign tumor and not amplication in normal ovary.Conclusion The amplification of ?ZNF217 is associated with ovarian cancer.Oncogenes ?ZNF217 maybe play a role in cell differentiation and indicate poorer survival in patients with ovarian cancer.
【Key words】 ovarian cancer; fluorescence in situ hybridization; ZNF217 gene;
- 【文献出处】 中华医学遗传学杂志 ,Chinese Journal of Medical Genetics , 编辑部邮箱 ,2006年06期
- 【分类号】R737.31
- 【被引频次】12
- 【下载频次】227