【摘要】 目的探讨参与DNA复制的两个重要基因CDT1和GMNN基因多态性与我国人群散发乳腺癌的关联。方法采用病例对照研究设计,研究对象包括427例乳腺癌患者及477名无肿瘤史的正常对照组。采用聚合酶链反应-限制性片段长度多态性方法测定CDT1838G/A,错配聚合酶链反应-限制性片段长度多态性方法测定GMNN387C/A的基因型。结果CDT1GG、GA和AA3种基因型以及GMNNCC、CA和AA3种基因型在病例组和对照组的频率分布差异无统计学意义(P值分别为0.619和0.793)。然而,分层分析发现,在有肿瘤家族史的人群中,CDT1GA+AA基因型可显著增加乳腺癌的危险性(调整OR:2.21,95%CI:1.20~4·09)。结论CDT1838G/A基因多态性和GMNN387C/A基因多态性与总的散发性乳腺癌无显著关联,但CDT1838G/A可能对于具有遗传背景的女性乳腺癌的易感性具有一定的作用。更多还原

【Abstract】 Objective To investigate the association of polymorphisms of CDT1 and GMNN gene, two important genes participating in DNA replication, with the risk of sporadic breast cancer. Methods [WTBZ]Using polymerase chain reaction-restriction fragment length polymorphism (PCR - RFLP) and the primer-introduced restriction analysis (PIRA)-PCR assay to genotype the CDT1 838G/A and GMNN 387C/A polymorphisms in a case-control study of 427 breast cancer cases and 477 cancer-free controls in a Chinese population. Results No significant association of the CDT1 838G/A and GMNN 387C/A polymorphisms with the risk of breast cancer was found (adjusted OR:1.16, 95% CI:0.88-1.54 for CDT1 GA+AA genotypes and adjusted OR:0.90, 95% CI:0.67-1.21 for GMNN CA+AA genotypes). However, in the stratified analyses, a significant association of CDT1 GA+AA genotypes with breast cancer risk among subjects with family history of cancer was found (adjusted OR:2.21, 95% CI:1.20-4.09). Conclusion These findings suggest that the CDT1 838G/A and GMNN 387C/A polymorphisms may not play a major role in the etiology of breast cancer, but CDT1 variant may have a potential role only in genetically susceptible women.更多还原