【摘要】 目的了解维生素D受体(vitaminDreceptor,VDR)基因(VDR)起始密码子(FokⅠ位点)和启动子区CDX2结合位点多态性与绝经后妇女钙剂补充对骨密度(bonemineraldensity,BMD)和骨转换指标作用的关系。方法200名受试对象(均为上海市汉族无亲缘关系的绝经后妇女)分为两组:高钙组(日服元素钙1000mg和维生素D400IU)和低钙组(日服元素钙300mg和维生素D300IU)各100名,期限1年。检测治疗前后BMD和骨转换指标,以及VDR基因FokⅠ位点和CDX2结合位点多态性。结果其中171名完成整个研究。研究人群FokⅠ基因型频率分布依次为Ff(48.0%)、FF(31.0%)和ff(21.0%);CDX2基因型频率分布依次为AG(56.7%)、GG(25.7%)和AA(17.6%),上述等位基因频率分布在整个人群或2亚组中均符合Hardy-Weinberg定律。无论在整个人群组、或是2亚组中,FokⅠ或CDX2各基因型间各部位BMD和各骨转换指标的基线值差异均无统计学意义;钙剂补充1年时,各部位BMD和骨转换指标的终点值和变化的百分数与FokⅠ或CDX2多态性均无相关性。结论高钙或低钙的补充对上海市汉族绝经后妇女BMD或骨转换指标的影响与VDR基因FokⅠ或CDX2多态性无相关性。更多还原

【Abstract】 Objective To investigate the association of polymorphisms of start codon (Fok Ⅰ site) and CDX2 binding site in vitamin D receptor gene (VDR) concerned with the effect of calcium supplementation on bone mineral density (BMD) and bone turnover markers of postmenopausal women. Methods Two hundreds unrelated postmenopausal women of Han ethnicity in Shanghai were randomly divided into 2 groups of 100 women: high calcium group (1000 mg element calcium and 400 units of vitamin D were given daily for 12 months) and low calcium group (300 mg element calcium and 300 units of vitamin D were given daily for 12 months). BMD and bone turnover markers were measured at baseline and 12 months after calcium supplementation. VDR gene Fok Ⅰ and CDX2 polymorphisms were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele-specific multiplex PCR, respectively. Results One hundred and seventy-one women completed 12-month study period. The frequency of VDR FokⅠ genotypes was 48.0 % for Ff, 31.0 % for FF, and 21.0 % for ff, and the frequency of CDX2 genotypes was 56.7 % for AG, 25.7% for GG, and 17.6% for AA. The frequencies distribution of FokⅠ and CDX2 alleles in the entire population or in two subgroups all followed the Hardy-Weinberg equilibrium. No significant difference of baseline BMD and bone turnover markers in Fok Ⅰ genotypes or CDX2 genotypes was observed in the entire population or in two subgroups. Moreover, regardless of calcium supplementation given for 12 months, no significant association was found between Fok Ⅰ or CDX2 polymorphisms and the endpoint values or percentage changes of any BMD and bone turnover markers in either high calcium group or low calcium group. Conclusion There is no significant relationship between VDR gene Fok Ⅰ or CDX2 polymorphisms and the effect of high or low doses calcium supplementation on BMD and bone turnover markers in Shanghai postmenopausal women of Han ethnicity.更多还原