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华南、华北人群HLA-DQB1等位基因多态性

With genechip technique to investigate HLA-DQB1 gene polymorphisms in south and north regions of China

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【作者】 王晖武大林申啊东陈春燕张文李丹刘亚琴

【Author】 WANG Hui~1, WU Da-lin~2, SHEN A-dong~3, CHEN Chun-yan~1, ZHANG Wen~1, LI Dan~4, LIU Ya-qin~3. ~1(Shenzhen Yi-Shengtang Biological LTD. Company, Shenzhen, Guangdong, 518054 P.R. China.); ~2(Center Laboratory of Southern Chinese Medical Science University, Guangzhou, Guangdong, 510515 P.R. China); ~3(Leukocyte Laboratory, Institute of Children, Peking Children′s Hospital, Beijing, 100045 P.R.China); ~4(Institute of Hemal Disease, People′s Hospital of Peking University, Beijing, 100034 P.R. China)

【机构】 深圳益生堂生物企业有限公司南方医科大学南方医院医学中心实验科北京儿童医院儿研所白细胞室深圳益生堂生物企业有限公司北京大学人民医院血液病研究所

【摘要】 目的从基因水平调查了中国华南、华北地区人群HLA-DQB1等位基因频率,并研究比较两地区人群HLA-DQB1多态性分布。方法采用深圳益生堂生物企业有限公司研制开发的“HLA-DQB1低分辨率分型基因芯片检测试剂盒”,应用聚合酶链反应-序列特异性引物+序列特异性寡核苷酸探针芯片检测技术,对700名南方地区的中国人和320名北方地区的中国人进行基因分型。结果鉴定了10个HLA-DQB1等位基因,获得了一组准确、科学的统计数据。结论得到了中国华南、华北地区人群HLA-DQB1等位基因频率差异的数据,证明中国人群HLA-DQB1*02,05,0601,0602,0603的分布南北差异有统计学意义(P<0.05),为疾病相关性研究、人文科学研究提供了可靠的遗传学数据。

【Abstract】 Objective To research and compare HLA-DQB1 gene frequency(GF) and polymorphism distribution between south and north population of Chinese in China. Methods Combining PCR-sequence specific primers(SSP) and sequence specific oligonucleotide probe(SSOP) techniques and DNA Microarray Kit for HLA-DQB1 Low Res Genotyping from Shenzhen Yi-Shengtang Biological LTD. Co. was used to type HLA-DQB1 gene polymorphisms of 700 individuals living in south China and 320 individuals in north China. Results We inspected 10 alleles of HLA-DQB1 and got a series of comprehensive and accurate statistic data. Conclusion It is tested that HLA-DQB1*02,05,0601,0602,0603 gene frequencies are different obviously(P<0.05) between south and north Chinese. And those data will be useful to kinds of reseach associated with disease relevant and anthropology research.

【基金】 国家863计划项目(2002AA2Z2032)~~
  • 【文献出处】 中华医学遗传学杂志 ,Chinese Journal of Medical Genetics , 编辑部邮箱 ,2006年02期
  • 【分类号】R394
  • 【被引频次】21
  • 【下载频次】176
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