【摘要】 目的研究中国云南彝族人群的群体遗传结构。方法选择9个短串联重复序列(shorttan-demrepeats,STR)位点(D3S1358、vWA、FGA、TH01、TPOX、CSF1PO、D5S818、D13S317、D7S820),采用STR复合扩增及荧光标记STR基因扫描技术,检测84名彝族无关个体血液样本。结果9个STR位点在84名云南彝族共检出69种等位片段,频率分布在0.0060~0.5060之间;检出164种基因型,频率分布在0.0119~0.4167之间。9个STR位点的基因型分布符合Hardy-Weinberg平衡定律(P>0.05)。9个位点多态信息量分布在0.5804~0.8777之间,杂合度分布在0.6507~0.8002之间,个体识别力分布在0.7976~0.9558之间,除TPOX,TH01两个位点低于0.5外,其余7个位点的非父排除率分布在0.5207~0.8386之间。Neighborjoining(NJ)法构建彝族与云南地区其他11个少数民族的系统进化树显示:彝族首先与白族、普米族、德昂族、阿昌族、独龙族、怒族聚在一起,然后与傈僳族、傣族相聚,最后与景颇族、苗族、纳西族相聚。结论获得了云南彝族9个STR位点的遗传多态数据,在人类群体遗传数据库建设、法医学亲权鉴定和个体识别研究及应用领域有重要价值。更多还原

【Abstract】 Objective To study the short teadem repeat(STR) genetics structure of a Chinese Yunnan Yi racial group. Methods Genetic distributions for nine STR loci were determined based on STR gene scan marked by fluorescence. Results Sixty-nine alleles and 164 kinds of genotypes were detected and identified from 84 unrelated Yi racial individuals. The corresponding gene and genotype frequencies were in 0.0060-0.5060 or 0.0119-0.4167 respectively. The expected and observed genotype frequencies of nine STR loci were in accordance with the Hardy-Weinberg equilibrium(P>0.05). The statistical analyses of nine STR loci showed that PIC was distributed in 0.5804-0.8777,H was in 0.6507-0.8002,DP was in 0.7976-0.9558, EPP was in 0.5207-0.8386, except TPOX and THO1 loci. Conclusion Above research data enrich the Chinese genetic database, and play an important role in Chinese genetic study and in forensic application.更多还原