【摘要】 目的研究白细胞介素13(IL-13)对红白血病细胞系 HEL 细胞分化作用及对 HEL 细胞转录因子 c-fos 表达的影响。方法用 RT-PCR 方法检测 HEL 细胞 IL-13受体α1、GPⅡb、vWF 和 c-fos基因 mRNA 表达,用 Western blot 和流式细胞术分别检测 IL-13受体α1、c-fos、GPⅡb和 vWF 蛋白水平的表达。结果 HEL 细胞表达 IL-13受体α1,用 IL-13(100 ng/ml)作用于 HEL 细胞,GPⅡb和 vWF 基因 mRNA 表达上调,实验组和对照组 GPⅡb/β-actin 吸光度比值分别为2.912,1.303,实验组为对照组的2.23倍(P<0.05);实验组和对照组 vWF/β-actin 吸光度比值分别为0.506,0.217,实验组为对照组的2.33倍(P<0.05)。流式细胞术分析结果显示,实验组 GPⅡb和 vWF 蛋白表达明显高于对照组。IL-13作用于 HEL 细胞30 min,c-fos 基因 mRNA 表达水平达高峰,IL-13作用 HEL 细胞60 min,c-fos 蛋白表达达高峰,30 min 组、60 min 组 c-fos/β-actin 吸光度比值高于其它各组(P<0.05)。结论IL-13可促进 HEL 细胞分化,并上调 c-fos 表达。更多还原

【Abstract】 Objective To study the effects of IL-13 on the differentiation and expression of transcrip- tion factor c-los of human erythroleukemia cell line(HEL)cells.Methods Reverse transcription polymer- ase chain reaction (RT-PCR) was used to observe the mRNA expression of IL-13 receptor α 1,GP Ⅱ b,vWF and c-los,and Western blot and cytometry were used to analyse their protein expression.Results IL-13 re- ceptor α 1 was expressed on HEL cells.IL-13 (100 ng/ml)up-regulated the mRNA expression of GP Ⅱ b and vWF.The ratio of luminous absorption (LA) of GP Ⅱb to β-actin bands (AB) was I.303 in control group,whereas was 2.912 in experiment group;being 2.23-fold higher than that in control group (P< 0.05).The ratio of LA to AB for vWF was 0.217 in control group,and 0.506 in experiment group;indica- ting a 2.33-fold increase in experiment group (P<0.05).The protein expression of GP Ⅱ b and vWF was significantly increased in experiment group,compared with that in control group.IL-13 inducing the inereased expression of e-los mRNA and protein of HEL cells peaked at 30 rain and 60 min,respectively.The ratio of LA to AB for e-los was also increased at 30 min and 60 min (P<0.05).Conclusion IL-13 prompts the differentiation of HEL cells and up-reglates the expression of c-fos.更多还原