【摘要】 目的探讨氧化还原态失衡与动脉粥样硬化的关系。方法应用高分辨超声技术检测颈动脉内膜增厚度,将167例住院患者分为斑块形成组(A组)79例,内膜中层增厚组(B组)52例,内膜中层正常组(C组)36例。取静脉血,测血浆还原型谷胱甘肽(GSH)与氧化型谷胱甘肽(GSSG),还原型辅酶Ⅱ(NADPH)与氧化型辅酶Ⅱ(NADP+),氧化型低密度脂蛋白(ox-LDL)和丙二醛(MDA)浓度。计算GSH/GSSG,NADP+/NADPH的氧化还原电位,该电位与ox-LDL行相关性分析。结果随内膜增厚(从C组到A组),GSH、GSH/GSSG渐次减低,GSH/GSSG氧化还原电位渐次升高(P<0.05);NADPH、NADPH/NADP+氧化还原态亦显示出与GSH、GSH/GSSG类似,但较弱的变化,氧化应激损伤产物ox-LDL、MDA随内膜增厚明显增加(P<0.05);GSH/GSSG氧化还原电位与ox-LDL明显正相关(P<0.05)。随着动脉内膜增厚、斑块形成,血浆GSH/GSSG氧化还原态向氧化方向明显偏移。结论氧化还原态失衡、向氧化方向偏移与脂质的氧化损伤、内膜增厚相关,与动脉粥样硬化的发生发展可能存在某种紧密的内在联系。更多还原

【Abstract】 Objective To explore the relationship between plasma redox status and atherosclerosis.Methods IVUS was performed in common carotid in the neck of 167 patients with heart diseases. Patients were divided into three groups: plaque-forming group (A,n=79),intima-thickening group (B,n=52) and control group (C,n=36). Plasma glutathione (reduced form GSH and oxidized form GSSG),nicotinamide adenine dinucleotide phosphate (reduced form NADPH and oxidized form NADP+),oxidized low density lipoprotein (ox-LDL) and malondialdehyde (MDA) were measured in all patients. The GSH/GSSG and NADPH/NADP+ redox potential were calculated according to Nernst equation,and correlation analysis performed.Results GSH and GSH/GSSG gradually reduced and GSH/GSSG redox potential gradually increased in proportion to the thickening of artery intima (from Group C to Group A,P<0.05). Similar but milder results were shown for NADPH and NADPH/NADP+ redox status. The products of oxidative stress ox-LDL and MDA also increased significantly (P<0.05) in proportion to the thickening of artery intima. GSH/GSSG redox potential is positively correlated to ox-LDL (P<0.05). The redox status shifted to oxidizing direction in proportion to the intima thickness.Conclusion The imbalance of plasma redox status deviating to oxidation might be implicated in oxidized injury of lipid,intima thickening and atherosclerosis progress.更多还原