【摘要】 目的通过检测胃腺癌组织中NF-κBp65、VEGF、Survivin的表达情况,探讨它们在胃腺癌发生发展过程中的作用以及相互作用关系。方法应用免疫组织化学SP法,检测73例胃癌组织及20例正常胃黏膜组织中NF-κBp65、VEGF、Survivin的表达情况,并分析其与胃癌临床病理特征之间的关系。结果NF-κBp65、VEGF、Survivin在胃腺癌中的表达率分别为58．9％、72．6％、46．6％,而在正常胃黏膜组织则表达不明显或不表达,两者差异有统计学意义(P<0．05)。NF-κBp65、VEGF、Survivin的表达与浸润深度、淋巴结转移、临床分期均密切相关(P<0．05),NF-κBp65的表达亦与胃腺癌分化程度有关(P<0．05)。NF-κBp65蛋白的表达与VEGF及Survivin蛋白的表达均呈显著正相关,VEGF与Survivin的表达亦呈显著正相关。结论NF-κBp65通过上调VEGF及Survivin的表达促进胃腺癌的发生发展;VEGF与Survivin在胃腺癌的发生发展中存在着协同作用。更多还原

【Abstract】 Objective To detect the protein expressions of NF-κBp65, VEGF and Survivin in gastric adenoma and to explore their roles in the pathogenesis of gastric adenocarcinoma as well as their interrelationships. Methods The protein expressions of NF-κBp65, VEGF and Survivin were detected by immu-nohistochemistry in 73 specimens of gastric adenocarcinoma tissues and 20 specimens of normal gastric mucosa cases and analyzed their relation with the clinicopathological characteristics of gastric cancer. Results The positive rates of NF-κBp65, VEGF and Survivin protein in gastric adenocarcinoma tissues were 58. 9% , 72. 6% and 46. 6% respectively, and those in normal gastric mucosa were not evident or present,the differences were significant between gastric adenocarcinoma tissues and normal gastric mucosa cases (P <0. 05). The expressions of NF-κBp65, VEGF and Survivin were closely related to the depth of infiltration, lymph node metastasis and clinical TNM stage (P < 0. 05) , and NF-κBp65 protein expression was also related to different grade of adenocarcinoma (P<0. 05). In addition,NF-κBp65 protein expression had positive correlations with both VEGF and Survivin expression ( P < 0. 05 ) , and the expression of VEGF was positively correlated with that of Survivin ( P <0. 05). Conclusion NF-κB contributes to the pathogenesis of gastric adenocarcinoma through up regulating the expression of VEGF and Survivin. VEGF and Survivin may have syn-ergetic effects in the pathogenesis of gastric adenocarcinoma.更多还原