【摘要】 目的探讨应用可吸收脱细胞猪主动脉基质制备人工食管进行食管替代的可行性。方法应用胰酶、TritonX-100制备脱细胞猪主动脉基质。切除实验犬5cm颈段食管,用两片脱细胞猪主动脉基质缝制的人工食管进行重建,观察存活情况和愈合过程。结果6只实验犬无死亡,发生吻合口瘘1只,1只在行内镜下扩张治疗时新生食管破裂死亡。病理结果显示术后2周时有疏松结缔组织及大量新生血管形成,4周时大部分人工食管被食管黏膜上皮覆盖,镜下见黏膜上皮细胞约5~8层,12周时上皮细胞分化至8~10层,有黏膜下腺体结构及肌肉组织。原人工食管大部分已吸收,无法用肉眼分辨人工和正常食管。结论可吸收猪胸主动脉脱细胞血管基质可作为理想的代食管材料,能较好诱导组织的再生,有较好的应用前景。更多还原

【Abstract】 Objective To investigate the feasability of resorbable porcine-derived, xenogeneic aortas acelullar tissue matrix (ACTM) to be used as scaffold for cervical esophageal defect repair in a dog model. Methods Esophageal segmental defect measuring 5cm in length were created by surgical resection in 6 mature Chinese mongrel health dogs, then the defect were repaired with aorta acelullar tissue matrix (ACTM). The animals were kept alive for periods. The healing process was observed. Results There no death after perioperative period, one dog had anastomotic leakage. one dog was died of esophagus rupture as the result of esophageal dilation. Submucosal tissue regeneration and abundant neovascularization were found at 2 weeks. The squamous epithelium covered the most part of grafts surface in dogs killed after 4 weeks. 12 weeks later the squamous epithelium comprising 8 to 12 layers, both esophageal glands and muscle tissue were found. The graft material itself was mostly absorbed and could not be identified by naked eyes in the dogs killed after 12 weeks. Conclusion ACTM show promise as an ideal treatment option for esophageal repair.更多还原