【摘要】 目的比较趋化因子受体CCR5、CCR7、CXCR3和CXCR6在丙肝患者肝内和外周血CD4+T淋巴细胞表面表达水平及其意义,同时进一步了解其与肝脏组织学炎症反应的关系。方法采用荧光标记抗趋化因子受体的单克隆抗体对肝内及外周血中CD4+T淋巴细胞表面的趋化因子受体进行染色后,采用9色11参数流式细胞仪LSRⅡ进行检测分析。结果(1)肝内CCR5+、CXCR3+或/和CXCR6+的CD4+T淋巴细胞频数高于外周血(P<0.001),而CCR7+CD4+T淋巴细胞频数低于外周血(P<0.001);(2)肝内CCR5+或CXCR6+的活性(CD38+)CD4+T淋巴细胞频数高于外周血(P<0.05);(3)肝内表达2种或2种以上趋化因子受体CCR5、CXCR3和CXCR6的CD4+T淋巴细胞频数明显高于外周血(P<0.001),而不表达或仅表达一种上述趋化因子受体CD4+T淋巴细胞频数明显低于外周血(P<0.001);(3)CCR5和CXCR6在肝内CD4+T淋巴细胞表面的表达有中等度相关;(4)肝内组织学炎症明显组表达趋化因子受体CCR5、CXCR3或CXCR6的CD4+T淋巴细胞频数高于炎症轻微组。结论趋化因子受体CCR5、CXCR3和CXCR6可能介导CD4+T淋巴细胞向肝内迁徙定植,并参与肝脏炎症的病理免疫学反应过程。更多还原

【Abstract】 Objective To provide a comprehensive comparison of expression of CCR5, CXCR3, CXCR6 and CCR7 on CD4+ T cells in liver and blood in HCV infection, and its correlation with histopathological inflammation activity of liver. Methods Lymphocytes purified from liver biopsy tissue and blood were simultaneously stained with six monoclonal antibodies and then analyzed by flow cytometry. Results (1) CCR5, CXCR3 and CXCR6 expressed more frequently on CD4+ T cells in liver than those in blood, while CCR7 only expressed at low levels on intrahepatic CD4+ T cells. (2) The frequency of intrahepatic active CD38+CD4+ T lymphocytes expressing CCR5 or CXCR6 appears to be slightly higher than inactive CD38-CD4+ T lymphocytes. (3) More intrahepatic CD4+ T lymphocytes(about 45%) co-expressed simultaneously two or three of those chemokine receptors, CCR5, CXCR3 and CXCR6, while most CD4+ T lymphocytes(about 90%) in PBMC expressed none or only one of those chemokine receptors. (4) CCR5 and CXCR6 moderately relevantly co-expressed on intrahepatic CD4+ T lymphocytes, while CCR5 and CXCR3, CXCR3 and CXCR6 relatively independently expressed on intrahepatic CD4+ T lymphocytes. (5) CCR5, CXCR3 and CXCR6 more frequently expressed on intrahepatic CD4+ T cells in those HCV infection with severe liver inflammation than those with mild to moderate liver inflammation. Conclusion CCR5, CXCR3 and CXCR6 are liver trafficking chemokine receptors which differently mediates homing CD4~+ T lymphocytes to liver, and may play an important role in the regulation of immunopathologic reaction during chronic HCV infection.更多还原