【摘要】 目的探讨转录因子 Twist 在人肝细胞肝癌、癌旁和肝硬化组织中的表达及其意义。方法利用免疫组化方法检测26例肝细胞肝癌标本(癌和癌旁组织)和10例肝硬化标本中 Twist 的表达情况;利用逆转录聚合酶链反应(RT-PCR)和 Western 蛋白印迹方法检测其中10例标本中的Twist mRNA 和其蛋白的表达情况,分析其临床病理意义。结果免疫组化显示:Twist 蛋白在肝癌、癌旁和肝硬化组织中的阳性率分别为84.6%、19.2%和20.0%,Twist 在肝癌组织中的表达明显强于癌旁或肝硬化组织(P<0.05);大多数癌旁和肝硬化组织无 Twist 表达,癌旁与肝硬化组织相比阳性率无差别(P<0.05)。与癌旁组织相比,Twist mRNA 和其蛋白在肝癌组织中表达明显上调(P<0.05)。结论 Twist 在肝细胞肝癌组织中的过高表达与肝癌的发生发展相关。更多还原

【Abstract】 Objective To investigate the expression and clinicopathological significance of transcription factor Twist in hepatocellular carcinoma(HCC),paraneoplastic and cirrhotic tissues.Method Immunohistochemistry was used to detect the expression of the Twist protein in 26 cases of HCC and paraneoplastic tissue and 10 cases of cirrhotic tissue.Meanwhile,the Twist mRNA and its protein were detected in 10 HCC tissues and 10 paraneoplastic tissues by RT-PCR and Western blot.And the expression differences and clinicopathological significances of the expression of Twist gene and its protein were analyzed.Results The positive rates of the Twist protein in HCC,paraneoplastic and cirrhotic tissues were 84.6%,19.2% and 20.0%,respectively.The positive rate of Twist in HCC was higher than that in paraneoplastic or cirrhosis tissues(P<0.05).Compared with in paraneoplastic tissues,the mRNA and protein expression of Twist were up-regulated in HCC by 2.52 and 2.13 times,respectively.Conclusion Twist has an inappropriate expression in hepatocellular carcinoma and it may play an important role in the tumorigenesis and progression of HCC.更多还原