【摘要】 目的观察姜黄素对前列腺癌细胞核转录因子抑制蛋白（IkBα）表达的影响,探讨姜黄素抑制前列腺癌细胞增殖的作用机制。方法分别用10、25、50、75和100μmol/L 浓度的姜黄素对雄激素依赖性及雄激素非依赖性前列腺癌细胞株 LNCaP 和 PC3进行干预,5、12和24 h 后采用噻唑蓝（MTT）比色法观察细胞增殖情况;采用流式细胞术测定24 h 后细胞周期变化;5 h 后 Western 印迹法检测细胞中 IkBα的表达。结果姜黄素显著抑制 LNCaP 及 PC3细胞的生长,呈剂量和时间依赖性;姜黄素将两种前列腺癌细胞阻滞于 G₂、M 期[LNCaP 与 PC3细胞,空白对照分别为（11.4±1.3）%与（17.3±1.7）%,100μmol/L 姜黄素作用后分别为（27.3±2.8）%与（33.4±4.0）%],从而诱导肿瘤细胞凋亡;姜黄素作用于 LNCaP 细胞后,细胞中 IkBα表达无变化（F=0.129,P>0.05）;但作用于 PC3细胞后,细胞中 IkBα的表达明显增强,呈现出显著的剂量依赖性（F=31.618,P<0.05）。结论姜黄素通过活化 IkBα在 PC3细胞中的表达发挥抑制 PC3细胞增殖的作用。对于 LNCaP 细胞,姜黄素可能通过抗氧化、抑制细胞内代谢产物形成等方式抑制 LNCaP 细胞增殖。更多还原

【Abstract】 Objective To investigate the eurcumin-induced the expression of IκBα in androgen- dependent（LNCaP）and androgen-independent（PC3）prostate cancer cells,and to study the mechanisms of curcumin on the proliferative inhibition of prostate cancer cells.Methods After LNCaP and PC3 cells were affected by 10,25,50,75,100 μmol/L curcumin respectively,the cell activity was assayed with methyl thiazolyl tetrazolium（MTT）method at 5,12 and 24 hours;Flow cytometry was adopted to observe the cell cycle of LNCaP and PC3 cells at 24 hours.After 5 hours,the expression of IκBα in LNCaP and PC3 cells was observed with Western blotting.Results Curcumin obviously suppressed the proliferation of LNCaP and PC3 cells in does-dependent and time-dependent manners.Curcumin could arrest the cell cycle of LNCaP and PC3 cells at G₂ M phase and then induce cell apoptosis.The expression of IκBαin LNCaP cells had no significant difference after using curcumin（F=0.129,P>0.05）.However,the expression of IκBα in PC3 cells increased gradually with the inducement of concentration-increased curcumin（F= 31.618,P<0.05）.Conclusions IκBα may play a role in the eureumin inducing apoptosis of PC3 cell, while the curcumin inducing apoptosis of LNCaP cells is by antioxidation and inhibiting metabolites formation in LNCaP cells.更多还原