【摘要】 目的研究肝细胞肝癌(hepatocellularcarcinoma,HCC)组织中上皮钙黏蛋白(E-cadherin)和血管内皮因子CD34的表达情况,探讨二者之间及与临床病理之间的关系及其对HCC患者预后的评价。方法应用免疫组织化学PV-6000两步法检测41例HCC组织标本中E-cadherin和CD34的表达,并与临床病理学指标和术后生存期进行比较分析。结果41例HCC组织中E-cadherin和CD34表达阳性率分别为48·8%和100%。E-cadherin蛋白的表达在大肝癌、侵袭性高危组和癌组织Edmondson分级Ⅲ、Ⅳ级的HCC患者中明显降低(χ2=4·1881、4·8118、6·2695;P均<0·05)。E-cadherin表达阴性的患者肝切除术后2年复发率较阳性表达组明显增加,5年生存率明显降低。CD34的表达与HCC患者年龄、侵袭性呈显著负相关(t=1·9371、1·9010;P<0·05),CD34阳性表达[微血管密度(MVD)≤40]组的2年复发率和5年生存率与MVD>40组相比,差异无统计学意义。E-cadherin与CD34的表达不相关。结论HCC组织中E-cadherin表达阴性的患者预示着较差的预后,CD34的阳性表达与肿瘤复发和患者生存率无相关关系,HCC组织中E-cadherin和CD34的共同表达无相关性。更多还原

【Abstract】 Objective To study the expression of E-cadherin and CD34 in the tissues of hepatocellular carcinoma(HCC), to discuss the relationship between them and the clinical pathology and evaluate the prognosis of HCC patients. Methods The expression of E-cadherin and CD34 in HCC tissues of 41 patients were examined by two-step methods of PV-6000 of immunohistochemistry. Clinical-pathological data, tumor recurrent rate and survival rate after hepatectomy were recorded and analyzed. Results The positive expression rate was observed in 48.78% for E-cadherin and 100% for CD34. The decreased E-cadherin expression were significantly associated with larger tumor, the high-dangerous group with invasion and poor differentiation of HCC tissues (χ~2=4.1881, 4.8118, 6.2695, P<0.05). In the group with negative-expression of E-cadherin, the percent of tumor recurrence within 2 years after hepatectomy was higher and the rate of 5 years survival was significantly lower than the positive-expressed group. A significant negative-correlation between the expression of CD34 and the patients′ age and the invasion of tumor (t=1.9371, 1.9010, P<0.05) were found. There was no relationship between the expression of E-cadherin and CD34 in HCC tissues. Conclusions The patient with a negative-expression of E-cadherin in HCC tissues has a poor prognosis. No relationship between the expression of CD34 and tumor recurrence and patients′ survival and no relationship between the expression of E-cadherin and CD34 was found.更多还原