【摘要】 目的通过测定子前期患者氧化应激产物和炎症标志物的水平,进一步研究子前期的发病机制。方法以53例子前期孕妇为研究组(其中轻度子前期32例、重度子前期21例),20例同期分娩的正常孕妇为对照组。检测血浆8-异前列腺素(8-isoprostane)、丙二醛(MDA)、氧化低密度脂蛋白(ox-LDL)和高敏C-反应蛋白(hs-CRP)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的浓度。结果(1)子前期患者血浆8-异前列腺素、MDA、ox-LDL的浓度分别为(153.07±44.84)pg/ml、(5.25±0.11)μmol/L、(772.04±151.00)μg/L,均高于对照组[(82.86±20.91)pg/ml、(4.67±0.38)μmol/L、(431.45±200.69)μg/L,P<0.01、>0.05和<0.05];(2)子前期患者血浆hs-CRP、IL-6、TNF-α水平分别为(2.17±1.29)mg/L、(26.49±12.73)pg/ml、(18.47±4.17)pg/ml均明显高于对照组[(1.46±1.00)mg/L、(13.35±5.01)pg/ml、(8.86±1.39)pg/ml,P均<0.01];(3)子前期患者8-异前列腺素与hs-CRP、IL-6、TNF-α呈显著正相关(P<0.01),而MDA及ox-LDL均与hs-CRP、IL-6、TNF-α无相关性(P>0.05)。结论氧化应激及炎症反应可能在子前期中发挥重要作用,干预氧化应激及炎症反应可能有利于控制子前期的发生和发展。更多还原

【Abstract】 Objective To investigate the changes of plasma markers of oxidative stress and inflammatory reaction in preeclampsia(PE)and to evaluate their clinical significance and interactions in the pathogenesis of PE.Methods A prospective study was conducted involving 53 women with PE(study group:including 32 cases of mild PE and 21 severe PE)and 20 normotensive women(control group)in the third trimester.The plasma concentrations of high-sensitive C-reactive protein(hs-CRP),interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α),malondialdehyde(MDA),ox-LDL and 8-isoprostane were determined.Results(1)The plasma concentrations of hs-CRP,IL-6,TNF-α,8-isoprostane and ox-LDL were significantly higher in the study group [(2.17±1.29)mg/L,(26.49±12.73)pg/ml,(18.47±4.17)pg/ml,(153.07±44.84)pg/ml,(772.04±151.00)μg/L,respectively] than those of the control(P<0.01 or P<0.05),while no significant difference were noted between the plasma levels of MDA between these two groups [(5.25±0.11)μmol/L vs(4.67±0.38)μmol/L,P>0.05].(2)The plasma levels of hs-CRP,IL-6,TNF-α,and 8-isoprostane were markedly elevated in the severe PE group [(2.84±1.76)mg/L,(27.27±18.38)pg/ml,(22.13±12.35)pg/ml,(163.99±51.01)pg/ml,respectively] than those in mild PE group(P<0.01 or P<0.05),while no significant difference was shown in the plasma levels of MDA and ox-LDL between the SPE and MPE group [(5.49±0.15)μmol/L vs(5.08±0.19)μmol/L,P>0.05;(809.29±211.83)μg/L vs(741.66±190.73)μg/L,P>0.05].(3)Plasma levels of 8-isoprostane were positively correlated with that of hs-CRP,IL-6,and TNF-alpha in PE women(r=0.618,P<0.01;r=0.689,P<0.01;r=0.712,P<0.01;respectively),but no relationship was found between the levels of MDA,ox-LDL and that of hs-CRP,IL-6,and TNF-α(r=0.168,P>0.05;r=0.113,P>0.05;and r=0.110,P>0.05,respectively).Conclusions Oxidative stress and inflammatory reaction play an important role in the pathogenesis of PE.Antioxidant and anti-inflammatory treatment may prevent the onset and progress of PE.更多还原