【摘要】 目的探讨激活素A(Activin A,ActA)对细胞滋养层细胞凋亡的调节作用。方法用ActA刺激无血清培养的7~8周人绒毛细胞滋养层细胞后,激光共聚焦和蛋白印迹分析检测凋亡信号通路相关蛋白的表达变化,TUNEL检测细胞凋亡情况。结果高浓度ActA(50 ng/ml)作用24 h后,人绒毛细胞滋养层细胞中TUNEL阳性信号显著增强;同时Caspase-3,-9表达量明显提高(对照组:0.13±0.048和0.26±0.004;50 ng/ml组:0.34±0.068和1.54±0.062;100 ng/ml组:0.45±0.091和0.58±0.008),Caspase-8表达亦略有增加;凋亡相关蛋白P53(对照组:5.2±1.02;50ng/ml组:12.3±1.91;100 ng/ml组:11.5±1.73)和Bax(对照组:0.09±0.021;50 ng/ml组:0.46±0.065;100 ng/ml组:0.68±0.142)表达水平显著增加。结论高浓度ActA可诱导人细胞滋养层细胞发生凋亡,其作用可能主要是通过细胞凋亡的线粒体途径来实现的。更多还原

【Abstract】 Objective To study the effect of activin A(ActA) on the regulation of cell apoptosis in human cytotrophoblasts in the early trimester.Methods Human cytotrophoblasts derived from placental villi at 7~8 gestational weeks were cultured in serum-free system,and treated with ActA.Cell apoptosis was detected by TUNEL,and the expression of apoptosis signaling molecules(including Caspase-3,-8 and-9) and apoptosis-related proteins(P53 and Bax) were detected by Confocol microscopy and Western Blot analysis.Results Treatment of ActA at high concentration(50 ng/ml) for 24 h significantly induced cell apoptosis in human cytotrophoblasts.The expressions of Caspase-3 and Caspase-9 were obviously up-regulated(control group:0.13±0.048 and 0.26±0.004;50 ng/ml group:0.34±0.068 and 1.54±0.062;100 ng/ml group:0.45±0.091 and 0.58±0.008),while the level of Caspase-8 increased slightly.Meanwhile,the expressions of P53(control group:5.2±1.02;50 ng/ml group:12.3±1.91;100 ng/ml group:11.5±1.73)and Bax(control group:0.09±0.021;50 ng/ml group:0.46±0.065;100 ng/ml group:0.68±0.142) protein in the cytotrophoblasts were strongly stimulated by ActA.Conclusions High concentration of ActA can induce cell apoptosis in human cytotrophoblasts which might be mediated by the mitochondria signaling pathway.更多还原