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单克隆抗体法与联苯胺法检测粪便隐血的对比

The comparison of testing fecal occult blood by one step test and benzidene test

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【作者】 冯彩莲陈莲香

【Author】 Feng Cailian,Chen Lianxiang.GuangZhou Xinhai Hospital,Guangdong Guangzhou,510300

【机构】 广东省广州市新海医院检验科广东省广州市新海医院检验科

【摘要】 目的单克隆抗体一步法与联苯胺法测定粪便隐血的方法学比较。方法分别用一步法和联苯胺法测定5种浓度的人血红蛋白液及不同动物的血红蛋白液和1031例病人的粪便隐血,对结果进行比较。结果一步法可测定出0.2μg/ml的微量血红蛋白,动物血试验为阴性,检测1031例患者的阳性率为41%。结论一步法的特异性、敏感性及准确性均比联苯胺法要高,对粪便中微量血红蛋白的检出具有十分重要的意义。

【Abstract】 Objective The comparison of testing fecal occult blood by One step test and Benzidine test.Methods Using One step test and Benzidine test to test 5 kind of concentration of hemoglobin solution and different animal hemoglobin solulions and 1031 patients’ fecal occult blood.Comparing of the results.Results One step can test 0.2μg/ml concentration of hemoglobin solution,animal hemoglobin test is negative,the positive rate of 1031 patients is 41%.Benzidine test can only test 2μg/ml concentration of hemogolbin solution and animal hemoglobin test is positive and the positive rate of 1031 patients is 35%.Conclusions One step test is more specificity and sensitivity and accuracy than Benzidine test.It has very important significance to test the minim hemoglobin of fecal.

  • 【文献出处】 中国现代医药杂志 ,Modern Medicine Journal of China , 编辑部邮箱 ,2006年09期
  • 【分类号】R446.6;R446.1
  • 【下载频次】47
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