【摘要】 目的探讨RANTES、MIP-1α趋化因子对人肝癌细胞侵袭转移能力的影响。方法应用Transwell小室研究RANTES、MIP-1α对人肝癌细胞侵袭力的影响,分别使用细胞-细胞黏附试验、细胞-基质黏附试验了解RANTES、MIP-1α预处理后人肝癌细胞黏附能力的变化。采用明胶酶谱法观察RANTES、MIP-1α刺激后人肝癌细胞基质金属蛋白酶活性改变。结果RANTES、MIP-1α等趋化因子能显著提高人肝癌细胞侵袭能力。黏附试验提示RANTES、MIP-1α可以增加人肝癌细胞与纤连蛋白和人脐静脉内皮细胞黏附。明胶酶谱显示RANTES、MIP-1α刺激后肝癌细胞基质金属蛋白酶活性显著增加。结论RANTES、MIP-1α等趋化因子可以增加人肝癌细胞侵袭转移能力。更多还原

【Abstract】 ObjectiveTo investigate the effects of RANTES and MIP-lαon invasive and metastatic potential of hepatoceilular cancer(HCC)cells.MethodsThe ability of migration,adhesion and degeneration of HCC cells was evaluated by ehemoinvasion assay,cell-cell adhesion assay,cell-matrix adhesion assay and gelatin zymography assay after ehemokines exposure.ResultsChemoinvasion assay showed that RANTES and MIP-lαcould increase the invasiveness of HCC cells.RANTES and MIP-lαpretreatment enhanced the binding of liver cancer cell lines to human umbilical vein endothelial ceils and fibronectin.In gelatin zymography assay,RANTES caused an increase in the activity of MMP-2 and MMP-9.Conclusion Both RANTES and MIP-lαcould increase invasive and metastatic potential of HCC cells.更多还原