【摘要】 目的利用维甲酸胎鼠模型,观察其骨骼畸形出现的情况,探讨骨骼畸形的发生机制。方法于孕期第10天经胃管给SD母鼠注入125 mg/kg体重全反式维甲酸,对照组仅予载体溶液。分娩前24 h剖腹取出胎鼠,实验组和对照组分别随机各取20只和10只胎鼠去除软组织,行骨软骨双重染色法,显微镜观察骨骼情况。结果实验组胎鼠体重(4．294±0．670)g与对照组(4．743±0．542)g比较,差异有统计学意义(P＜0．05)。对照组未见骨骼畸形。维甲酸组有18只(90%)胎鼠可见明显的全身骨骼畸形,最常见的畸形分别是长骨畸形(80%)、腕掌跗跖骨缺如(80%)、胸廓外形异常(80%)、椎体畸形(75%)、骨盆畸形(70)和脊椎裂(65%)。结论维甲酸有较强的胎鼠骨骼致畸作用,这种作用是多部位的。维甲酸大鼠是研究骨骼先天性畸形的实用工具模型。更多还原

【Abstract】 Objective To observe the skeletal malformations in fetal rats treated with retinoic acid and study the pathogenic mechanism of skeletal malformations.Methods Time-mated pregnant SD rats were fed either 125 mg/kg of all-trans-retinoic acid or vehicle on gestational day 10,and the fetuses were removed near term.Twenty fetuses from experimental group and 10 from control group were ran- domly selected.Skeletal anatomy was studied after alcian blue and alizarin red staining.Results Body weight of experimental group was much less than that of control group(P＜0.05).All control fetuses were normal,whereas 18 of 20 experimental fetuses had skeletal malformations.The most common mal- formations were long bone malformations(80%),absence of carpal or metacarpal or tarsal or metatarsal or digits(80%),malformation of compages thoracis(80%),vertebrae malformations(75%)and spinal bifida(65%).Conclusion Retinoic acid has a strong and multi-regional teratogenic effect on the skele- ton of fetal rat.The rat model of retinoic acid is a useful tool for investigation of congenital skeletal mal- formations.更多还原