【摘要】 目的观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)对裸鼠胃癌移植瘤的生长抑制作用,探讨死亡相关蛋白3(DAP3)与其的关系。方法建立人胃癌细胞株BGC-823裸鼠移植瘤模型；观察不同剂量的TRAIL(0、1、5、10mg/kg体重)对移植瘤的生长抑制作用；流式细胞仪测定细胞周期及凋亡情况；Western blot和逆转录-聚合酶链反应(RT-PCR)检测DAP3蛋白和DAP3 mR- NA表达情况。结果5 mg/kg体重和10 mg/kg体重两组的瘤重分别为(2．57±0．35)g和(2．49±0．26)g,均低于1 mg/kg体重组(3．23±0．32)g和对照组(3．57±0．47)g,前两组的细胞增殖指数分别为(36．69±5．16)%和(39．69±6．13)%,低于对照组(48 06±6．99)%；Western blot和RT- PCR法分别测得前两组中DAP3蛋白和mRNA的表达高于1 mg/kg体重组和对照组。结论TRAIL可有效抑制裸鼠胃癌移植瘤的生长,其作用机制可能与诱导DAP3表达有关。更多还原

【Abstract】 Objective To observe the effect of tumor necrosis factor-related apoptosis-inducing lig- and(TRAIL)on human gastric cancer BGC-823 and to evaluate its relation with death associated protein 3(DAP3).Methods Nude mice model implanted sub-cutaneously with BGC-823 was established.Dif- ferent doses of TRAIL(0,1,5 and 10 mg/kg)were administrated intraperitoneally.The rate of tumor inhibition and apoptosis of tumor were measured.The expression of DAP3 protein and mRNA was detect- ed by Western blot and RT-PCR method respectively.Results The tumor weight of the 5 mg/kg group and 10 mg/kg group was(2.57±0.35)g and(2.49±0.26)g respectively,both significantly less than that of control group.The expression of DAP3 protein and mRNA was increased in 5 mg/kg and 10 mg/ kg group.Conclusion TRAIL could inhibit human gastric cancer BGC-823 implanted in nude mice.Its mechanism might be related to enhanced expression of DAP3.更多还原