【摘要】 目的观察淫羊藿甙(ICA)抑制胃癌细胞黏附、移动及侵袭的效应,探讨ICA对其恶性表型的逆转作用。方法常规培养人胃癌细胞株SGC-7901细胞；200 mg/L淫羊藿甙作用细胞48 h后,检测黏附率、迁移速度、侵袭力(Transwell法)及蛋白激酶A(PKA)活性。结果200 mg/L ICA处理48 h后,SGC-7901细胞在Ⅰ型胶原上黏附40 min时和80 min时,其黏附率分别为34．72%、58．12%,均明显低于对照组的40．31%、68．42%(P＜0．05),并且这种黏附抑制作用随时间的延长更为明显；细胞迁移速度[(1．27±0．22)μm/h]和细胞的侵袭力[(41．67±4．80)细胞数/每视野],均较对照组明显降低[(2．28±0．39)μm/h；(137．25±7．25)细胞数/每视野](P＜0．01)；但PKA活性由(0．6±0．1)U/ml增高到(0．9±0．1)U/ml(P＜0．01)。结论ICA抑制SGC-7901细胞黏附、移动及侵袭,增加了细胞内PKA活性,从而发挥逆转其恶性表型的作用。更多还原

【Abstract】 Objective To explore the inhibitive effect and mechanism of icariin(ICA)on adhe- sion and migration of SGC-7901 cells.Methods Adhesive ratio was detected by adhesion assay,migration rate was measured by wound healing assay,and invasion ability was detected by Transwell assay.PKA ac- tivity was measured by PKA activity assay.Results After SGC-7901 cells were treated with ICA of 200 mg/L for 48 h,adhesive ratio at 40 min and 80 min was 34.72% and 58.12% respectively,which was markedly reduced as compared with the control group(40.31% and 68.42% respectively,P＜0.05). The migration rate(1.27±0.22)μm/h and the invasion ability(41.67±4.80 cells/per view)was sig- nificantly decreased as compared with the control group(2.28±0.39μm/h,(137.25±7.25)cells/per view (P＜0.01).Whereas PKA activity was significantly increased in ICA group(0.9±0.1)U/ml as compared with control group(0.6±0.1)U/ml,P＜0.01.Conclusion ICA can inhibit adhesion,mi- gration and invasion ability of SGC-7901 cells effectively as well as increase their PICA activity.ICA may play anti-metastatic roles.更多还原